Mouse FGF-15 Alexa Fluor® 750-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6755S
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry
Label
Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant mouse FGF-15
Ser24-Lys218
Accession # O35622
Ser24-Lys218
Accession # O35622
Specificity
Detects mouse FGF-15 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) FGF-19 is observed and less than 5% cross-reactivity with rhFGF-22 and rhFGF-23 is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Mouse FGF-15 Alexa Fluor® 750-conjugated Antibody
Application
Recommended Usage
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: FGF-15
FGF-15 (Fibroblast growth factor 15) is a 25 kDa, secreted, nonglycosylated polypeptide that is a member of the FGF-15/21/23 subfamily, FGF family of growth factors. It is synthesized by ileal epithelium, colonic myofibroblasts, and select areas of the developing brain. FGF-15 acts on hepatocytes and reduces CYP7A1 levels, thus blocking bile acid production. It also promotes colonic epithelial migration, and in the embryonic brain, inhibits over-production of neuronal precursors. FGF-15 is synthesized as a 218 amino acid (aa) precursor that contains a 25 aa signal sequence and a 182 aa mature region (aa 26-218; 23 kDa predicted MW). Mature FGF-15 contains a characteristic 3-dimensional beta-trefoil structure, plus a unique Cys pattern that contributes to stability. There are two potential splice forms. One shows a 23 aa substitution for aa 120-218, while a second shows a 41 aa substitution for aa 120-218. Over aa 23-200, mouse FGF-15 shares 96% aa identity with rat FGF-15. Although mouse FGF-15 is considered the ortholog to human FGF-19, the two molecules share only 54% aa identity over aa 23-200, and are produced by different cell types (intestinal epithelium in mouse, hepatocytes in human).
Long Name
Fibroblast Growth Factor 15
Alternate Names
FGF-19, FGF15
Entrez Gene IDs
14170 (Mouse)
Gene Symbol
FGF15
UniProt
Additional FGF-15 Products
Product Specific Notices for Mouse FGF-15 Alexa Fluor® 750-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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