Mouse Epigen Alexa Fluor® 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB1127R
U.S. Patent No. 6,150,502
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 187240
Product Specifications
Immunogen
E. coli-derived recombinant mouse Epigen
Leu53-Ala103
Accession # Q924X1.1
Leu53-Ala103
Accession # Q924X1.1
Specificity
Detects mouse Epigen in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Amphiregulin, rhEGF, recombinant mouse (rm) Epiregulin, rhHB-EGF, rhTGF-alpha, rhHeregulin-alpha, rhHeregul
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse Epigen Alexa Fluor® 647-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Epigen
Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily (1-3). Mouse Epigen cDNA encodes a 152 amino acid (aa) residue type I transmembrane precursor protein with a 23 aa putative signal peptide, an 86 aa extracellular domain, a 21 aa transmembrane domain and a 22 aa cytoplasmic domain (2, 3). The extracellular domain (aa 24-109) contains a pattern of six cysteines typical of EGF family proteins (1-3). Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form (3, 4). Cleavage of Epigen by TACE/ADAM17 has been demonstrated (4). The mature, shed form of mouse Epigen (aa 53-103) shares 92%, 96% and 90% aa sequence identity with human, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGF-alpha and epiregulin (2). Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands (3, 5). Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate (3). Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1 (2, 3). The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion (3).
Alternate Names
EPGN
Gene Symbol
EPGN
UniProt
Additional Epigen Products
Product Specific Notices for Mouse Epigen Alexa Fluor® 647-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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