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Mouse EphB4 Antibody

EphB4 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Key Product Details

Species Reactivity: Mouse
Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EphB4
Leu16-Ala539
Accession # P54761
Applications: CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label: Unconjugated
Source: Polyclonal Goat IgG
Specificity:

Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.

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Citations for Mouse EphB4 Antibody (30)

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Customer Reviews (2)

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  • Mouse EphB4 Antibody
    Name: Anonymous
    Application: ICC/IF
    Sample Tested: E12.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/16/2020
    Dilution used - 1:200. The staining was done on an E12.5 transverse (4% PFA fixed) mouse section using standard IF techniques.Used 1% BSA for blocking for 1 hour before adding the primary antibody.The results are hit or miss for me, because I have noticed expression on non target tissue (Sometimes it doesn't appear, sometimes it does).Attached pictures shows a artery (smaller circle) and a vein (bigger circle), and while the EphB4 expression on the vein is very good (as it is supposed to express there), there seems to be some non-specific expression on the adjacent artery as well.
    Mouse EphB4 Antibody Immunocytochemistry/Immunofluorescence AF446
  • Mouse EphB4 Antibody
    Name: Anonymous
    Application: WB
    Sample Tested: embryos
    Species: Zebrafish
    Verified Customer | Posted 11/13/2020
    Mouse EphB4 Antibody Western Blot AF446

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Product Documents

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