Mouse EphB4 Antibody

Name: Mouse EphB4 Antibody
Brand: R&D Systems
SKU: AF446
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF446

Citations (33)

(2)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse EphB4 Leu16-Ala539 Accession # P54761
Applications:	CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.

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Applications for Mouse EphB4 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse EphB4 Fc Chimera (Catalog # 446-B4)
Flow Cytometry	2.5 µg/10⁶ cells	MCF‑7 human breast cancer cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse EphB4 Antibody

	Immunohistochemistry EphB4 in Mouse Embryo. EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Item	Value
References	Eph Nomenclature Committee [letter] (1997) Cell 90:403. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name	Eph Receptor B4
Entrez Gene IDs	2050 (Human); 13846 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, HTKephrin receptor EphB4, Htk, Mdk2, Myk1, Tyro11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK, ephrin type-B receptor 4, hepatoma transmembrane kinase, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3

Citations for Mouse EphB4 Antibody (33)

Showing 1 - 10 of 33 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse EphB4 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IF

AE Lewis et al. (2022-01-01), Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 38(11):110510.
PMID: 35294885
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

H Maruoka et al. (2022-01-01), Histochemical examination of blood vessels in murine femora with intermittent PTH administration Journal of oral biosciences, 0(0).
PMID: 35584768
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

S Kohara et al. (2022-01-01), Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 10(12).
PMID: 36551990
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

J Alam et al. (2021-01-01), Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary Reproductive biology, 21(3):100511.
PMID: 33991765
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

C Donato et al. (2020-01-01), Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 32(10):108105.
PMID: 32905777
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Neal et al. (2019-01-01), Venous identity requires BMP signalling through ALK3 Nat Commun, 10(1):453.
PMID: 30692543
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

S Payne et al. (2019-01-01), Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 10(1):3276.
PMID: 31332177
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

G Luxán et al. (2019-01-01), Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 8(0).
PMID: 31782728
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

E Groppa et al. (2018-01-01), EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 0(0).
PMID: 29643120
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

H Toray et al. (2017-01-01), Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice Biomed. Res., 38(2):123-134.
PMID: 28442663
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

M Wang et al. (2017-01-01), Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase J. Vasc. Surg., 65(1):179-189.
PMID: 26817610
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2016-01-01), EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis J Clin Invest, 0(0).
PMID: 27400125
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

(2016-01-01), EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 7(0):12329.
PMID: 27470974
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

(2016-01-01), Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol J Exp Clin Cancer Res, 35(1):118.
PMID: 27448598
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Zhang et al. (2015-01-01), EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 6(0):6625.
PMID: 25865237
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Valverde-Franco G et al. (2015-01-01), The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice. Am J Pathol, 185(2):335-46.
PMID: 25453723
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Nguyen T et al. (2015-01-01), EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance. Stem Cells, 33(9):2838-49.
PMID: 26033476
Lange A et al. (2014-01-01), Sox17 is required for normal pulmonary vascular morphogenesis. Dev Biol, 387(1):109-20.
PMID: 24418654
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

Hughes A et al. (2014-01-01), Single-cell western blotting. Nat Methods, 11(7):749-55.
PMID: 24880876
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Lindskog et al. (2014-01-01), Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals. Development, 141(5):1120-8.
PMID: 24550118
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Kawano H et al. (2012-01-01), A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 42(6):1562-72.
PMID: 22622783
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Muto A et al. (2011-01-01), Eph-B4 prevents venous adaptive remodeling in the adult arterial environment. J. Exp. Med., 208(3):561-75.
PMID: 21339325
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Foster KE et al. (2010-01-01), EphB-ephrin-B2 interactions are required for thymus migration during organogenesis. Proc. Natl. Acad. Sci. U.S.A., 107(30):13414-9.
PMID: 20616004
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Lin FJ et al. (2010-01-01), Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 120(5):1694-707.
PMID: 20364082
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Wang Y et al. (2010-01-01), Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Nature, 465(7297):483-6.
PMID: 20445537
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Xie J et al. (2009-01-01), Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice. Am. J. Pathol., 175(3):1315-27.
PMID: 19644017
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Allan EH et al. (2008-01-01), EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. J. Bone Miner. Res., 23(8):1170-81.
PMID: 18627264
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Kim YH et al. (2008-01-01), Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development, 135(22):3755-64.
PMID: 18952909
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Zhang J et al. (2008-01-01), Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice. Biol. Reprod., 79(3):450-8.
PMID: 18463357
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation

Yang NY et al. (2006-01-01), The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 281(43):32574-86.
PMID: 16950769
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Ogawa K et al. (2006-01-01), EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 119(0):559-70.
PMID: 16443753
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Martiny-Baron G et al. (2004-01-01), Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia, 6(3):248-57.
PMID: 15153337
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Fuller T et al. (2003-01-01), Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 116(0):2461-70.
PMID: 12734395

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Customer Reviews for Mouse EphB4 Antibody (2)

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Mouse EphB4 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: E12.5 mouse embryo fixed in 4% PFA

Species: Mouse

Verified Customer | Posted 12/16/2020

Dilution used - 1:200. The staining was done on an E12.5 transverse (4% PFA fixed) mouse section using standard IF techniques.Used 1% BSA for blocking for 1 hour before adding the primary antibody.The results are hit or miss for me, because I have noticed expression on non target tissue (Sometimes it doesn't appear, sometimes it does).Attached pictures shows a artery (smaller circle) and a vein (bigger circle), and while the EphB4 expression on the vein is very good (as it is supposed to express there), there seems to be some non-specific expression on the adjacent artery as well.
Mouse EphB4 Antibody

Name: Anonymous

Application: WB

Sample Tested: embryos

Species: Zebrafish

Verified Customer | Posted 11/13/2020

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Mouse EphB4 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse EphB4 Antibody

Scientific Data Examples for Mouse EphB4 Antibody

Immunohistochemistry

Formulation, Preparation and Storage

Plan Your Experiments

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Background: EphB4

Citations for Mouse EphB4 Antibody (33)

Customer Reviews for Mouse EphB4 Antibody (2)

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Mouse EphB4 Antibody

Mouse EphB4 Antibody

Product Documents for Mouse EphB4 Antibody

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