Mouse EphB4 Antibody
R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity: | Mouse |
Immunogen: |
Mouse myeloma cell line NS0-derived recombinant mouse EphB4 Leu16-Ala539 Accession # P54761 |
Applications: | CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot |
Label: | Unconjugated |
Source: | Polyclonal Goat IgG |
Specificity: |
Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed. |
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Citations for Mouse EphB4 Antibody (33)
Citations are publications that use Bio-Techne products. Selected citations for Mouse EphB4 Antibody include:
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Species: Mouse
Sample Types: Whole Tissue
Applications: IFAE Lewis et al. (2022-01-01), Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 38(11):110510.
PMID: 35294885 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Maruoka et al. (2022-01-01), Histochemical examination of blood vessels in murine femora with intermittent PTH administration Journal of oral biosciences, 0(0).
PMID: 35584768 -
Species: Mouse
Sample Types: Whole Tissue
Applications: BioassayS Kohara et al. (2022-01-01), Eph/Ephrin Promotes the Adhesion of Liver Tissue-Resident Macrophages to a Mimicked Surface of Liver Sinusoidal Endothelial Cells Biomedicines, 10(12).
PMID: 36551990 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCJ Alam et al. (2021-01-01), Expression and localisation of ephrin-B1 and EphB4 in steroidogenic cells in the naturally cycling mouse ovary Reproductive biology, 21(3):100511.
PMID: 33991765 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotC Donato et al. (2020-01-01), Hypoxia Triggers the Intravasation of Clustered Circulating Tumor Cells Cell Rep, 32(10):108105.
PMID: 32905777 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Neal et al. (2019-01-01), Venous identity requires BMP signalling through ALK3 Nat Commun, 10(1):453.
PMID: 30692543 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrS Payne et al. (2019-01-01), Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 10(1):3276.
PMID: 31332177 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotG Luxán et al. (2019-01-01), Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 8(0).
PMID: 31782728 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrE Groppa et al. (2018-01-01), EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 0(0).
PMID: 29643120 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Toray et al. (2017-01-01), Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice Biomed. Res., 38(2):123-134.
PMID: 28442663 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotM Wang et al. (2017-01-01), Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase J. Vasc. Surg., 65(1):179-189.
PMID: 26817610 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr(2016-01-01), EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis J Clin Invest, 0(0).
PMID: 27400125 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot(2016-01-01), EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 7(0):12329.
PMID: 27470974 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P(2016-01-01), Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol J Exp Clin Cancer Res, 35(1):118.
PMID: 27448598 -
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western BlotZhang et al. (2015-01-01), EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 6(0):6625.
PMID: 25865237 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PValverde-Franco G et al. (2015-01-01), The in vivo effect of prophylactic subchondral bone protection of osteoarthritic synovial membrane in bone-specific Ephb4-overexpressing mice. Am J Pathol, 185(2):335-46.
PMID: 25453723 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotNguyen T et al. (2015-01-01), EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance. Stem Cells, 33(9):2838-49.
PMID: 26033476 -
Lange A et al. (2014-01-01), Sox17 is required for normal pulmonary vascular morphogenesis. Dev Biol, 387(1):109-20.
PMID: 24418654 -
Species: Rat
Sample Types: Cell Lysates
Applications: Western BlotHughes A et al. (2014-01-01), Single-cell western blotting. Nat Methods, 11(7):749-55.
PMID: 24880876 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCLindskog et al. (2014-01-01), Molecular identification of venous progenitors in the dorsal aorta reveals an aortic origin for the cardinal vein in mammals. Development, 141(5):1120-8.
PMID: 24550118 -
Species: Mouse
Sample Types: Cell Lysates
Applications: ImmunoprecipitationKawano H et al. (2012-01-01), A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 42(6):1562-72.
PMID: 22622783 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCMuto A et al. (2011-01-01), Eph-B4 prevents venous adaptive remodeling in the adult arterial environment. J. Exp. Med., 208(3):561-75.
PMID: 21339325 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryFoster KE et al. (2010-01-01), EphB-ephrin-B2 interactions are required for thymus migration during organogenesis. Proc. Natl. Acad. Sci. U.S.A., 107(30):13414-9.
PMID: 20616004 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PLin FJ et al. (2010-01-01), Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development. J. Clin. Invest., 120(5):1694-707.
PMID: 20364082 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PWang Y et al. (2010-01-01), Ephrin-B2 controls VEGF-induced angiogenesis and lymphangiogenesis. Nature, 465(7297):483-6.
PMID: 20445537 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrXie J et al. (2009-01-01), Endothelial-specific expression of WNK1 kinase is essential for angiogenesis and heart development in mice. Am. J. Pathol., 175(3):1315-27.
PMID: 19644017 -
Species: Mouse
Sample Types: Cell Lysates
Applications: Western BlotAllan EH et al. (2008-01-01), EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts. J. Bone Miner. Res., 23(8):1170-81.
PMID: 18627264 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrKim YH et al. (2008-01-01), Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development, 135(22):3755-64.
PMID: 18952909 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-PZhang J et al. (2008-01-01), Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice. Biol. Reprod., 79(3):450-8.
PMID: 18463357 -
Species: Mouse
Sample Types: Cell Lysates
Applications: ImmunoprecipitationYang NY et al. (2006-01-01), The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization. J. Biol. Chem., 281(43):32574-86.
PMID: 16950769 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCOgawa K et al. (2006-01-01), EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 119(0):559-70.
PMID: 16443753 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMartiny-Baron G et al. (2004-01-01), Inhibition of tumor growth and angiogenesis by soluble EphB4. Neoplasia, 6(3):248-57.
PMID: 15153337 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotFuller T et al. (2003-01-01), Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells. J. Cell. Sci., 116(0):2461-70.
PMID: 12734395
There are no citations that match your criteria.
Customer Reviews for Mouse EphB4 Antibody (2)
Customer Images
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Application: ICC/IFSample Tested: E12.5 mouse embryo fixed in 4% PFASpecies: MouseVerified Customer | Posted 12/16/2020Dilution used - 1:200. The staining was done on an E12.5 transverse (4% PFA fixed) mouse section using standard IF techniques.Used 1% BSA for blocking for 1 hour before adding the primary antibody.The results are hit or miss for me, because I have noticed expression on non target tissue (Sometimes it doesn't appear, sometimes it does).Attached pictures shows a artery (smaller circle) and a vein (bigger circle), and while the EphB4 expression on the vein is very good (as it is supposed to express there), there seems to be some non-specific expression on the adjacent artery as well.
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Application: WBSample Tested: embryosSpecies: ZebrafishVerified Customer | Posted 11/13/2020
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