Mouse EphB4 Alexa Fluor™ Plus 488-conjugated Antibody

Name: Mouse EphB4 Alexa Fluor™ Plus 488-conjugated Antibody
Brand: R&D Systems
SKU: AF446AFP488

R&D Systems, part of Bio-Techne | Catalog # AF446AFP488

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Mouse
Label:	Alexa Fluor Plus 488 (Excitation=493 nm, Emission=518 nm)
Specificity:	Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.

View Full Technical Details for Mouse EphB4 Alexa Fluor™ Plus 488-conjugated Antibody

Technical Data
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Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Formulation	Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

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Background: EphB4

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Item	Value
References	Eph Nomenclature Committee [letter] (1997) Cell 90:403. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name	Eph Receptor B4
Entrez Gene IDs	2050 (Human); 13846 (Mouse)
Alternate Names	EC 2.7.10, EC 2.7.10.1, EPH receptor B4, EphB4, HTKephrin receptor EphB4, Htk, Mdk2, Myk1, Tyro11, Tyrosine-protein kinase TYRO11, Tyrosine-protein kinase receptor HTK, ephrin type-B receptor 4, hepatoma transmembrane kinase, soluble EPHB4 variant 1, soluble EPHB4 variant 2, soluble EPHB4 variant 3

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Mouse EphB4 Alexa Fluor™ Plus 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # AF446AFP488

Key Product Details

Applications

Formulation, Preparation and Storage

Reconstitution Calculator

Background: EphB4

Customer Reviews

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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