Mouse E-Selectin/CD62E Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5751
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 96419
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin
Trp22-Pro557
Accession # Q00690
Trp22-Pro557
Accession # Q00690
Specificity
Detects mouse E-Selectin in ELISAs and Western blots. Does not cross-react with recombinant human (rh) E‑Selectin, rmL-Selectin, or rhP-Selectin.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse E-Selectin/CD62E Antibody
Mouse E-Selectin / CD62E ELISA Standard Curve
Recombinant Mouse E-Selectin/CD62E Fc Chimera (Catalog # 575-ES) was serially diluted and captured by Rat Anti-Mouse E-Selectin/CD62E Monoclonal Antibody (Catalog # MAB5751) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse E-Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Mouse E-Selectin/CD62E Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES)
under non-reducing conditions only
Sample: Recombinant Mouse E‑Selectin/CD62E Fc Chimera (Catalog # 575-ES)
under non-reducing conditions only
Mouse E-Selectin/CD62E Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Alternate Names
CD62E, ELAM1, LECAM2, SELE
Gene Symbol
SELE
UniProt
Additional E-Selectin/CD62E Products
Product Documents for Mouse E-Selectin/CD62E Antibody
Product Specific Notices for Mouse E-Selectin/CD62E Antibody
For research use only
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