Mouse DLL4 Antibody

Name: Mouse DLL4 Antibody
Brand: R&D Systems
SKU: AF1389
Price: 152 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1389

Citations (29)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse DLL4 Ser28-Pro525 Accession # BAB18580
Applications:	Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse DLL4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human DLL4 is observed.

View Full Technical Details for Mouse DLL4 Antibody

Applications for Mouse DLL4 Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	See below
Immunohistochemistry	5-15 µg/mL	See below
Immunocytochemistry	5-15 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse DLL4 Antibody

	Western Blot Detection of Mouse DLL4 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DLL4 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry DLL4 in bEnd.3 Mouse Cell Line. DLL4 was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry DLL4 in Mouse Embryo. DLL4 was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse DLL4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1389) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to developing vasculature. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: DLL4

Delta-Like protein 4 (DLL4) is a type I membrane protein belonging to the Delta/Serrate/Lag2 (DSL) family of Notch ligands (1). Notch signaling is an evolutionarily conserved pathway that controls cell fate and is required in multiple developmental processes including vascular development, hematopoiesis, somatogenesis, myogenesis, and neurogenesis (2-4). Dysregulation in the Notch pathway is associated with various human diseases. In mammals, four Notch homologs (Notch 1 to 4) and five ligands (DLL 1, 3 and 4, Jagged 1 and 2) have been identified. Notch ligands are transmembrane proteins with a DSL motif necessary for Notch binding, tandem EGF repeats, a transmembrane region and a short intracellular domain (ICD). Notch ligands are categorized into two subfamilies based on the presence of an extracellular cysteine-rich domain and insertions that interrupt some EGF repeats in the Jagged but not the Delta ligand family. Interactions of Notch receptors with their ligands results in reciprocal Regulated Intramembrane Proteolysis (RIP) (4). RIP is a mechanism for transmembrane signal transduction that involves the sequential processing by A Disintegrin Metalloprotease (ADAM) and then by Presenilin/ gamma -Ssecretase, resulting in shedding of the extracellular domains and the generation of the soluble ICD signaling fragments, respectively. The Notch ICD translocates to the nucleus and interacts with transcriptional coactivators, resulting in the transcription of target genes. The ICDs of the Notch ligands have also been shown to translocate to the nucleus where they may have a signaling function (5, 6). DLL4 is expressed highly and selectively within the arterial endothelium and has been shown to function as a ligand for Notch 1 and Notch 4. Human and mouse DLL4 share 86% amino acid sequence identity (1).

Item	Value
References	Shutter, J.R. et al. (2000) Genes Dev. 14:1313. Iso, Tatsuya et al. (2002) Arterioscler. Thromb. Vasc. Biol. 23:543. Walker, L. et al. (2001) Stem Cells 19:543. Baron, M. (2002) Semin. Cell Dev. Biol. 14:113. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751. Bland, C.E. et al. (2003) J. Biol. Chem. 278:13607.
Long Name	Delta-like 4
Entrez Gene IDs	54567 (Human); 54485 (Mouse); 100152163 (Porcine)
Alternate Names	DLL4, Delta 4 precursor, Drosophila Delta homolog 4, MGC126344, delta 4, delta ligand 4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, delta4, hdelta2, notch ligand DLL4, notch ligand delta-2

Citations for Mouse DLL4 Antibody (29)

Showing 1 - 10 of 29 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse DLL4 Antibody include:

Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

C Xu et al. (2022), Induction of osteogenesis by bone-targeted Notch activation Elife, 11(0).
PMID: 35119364
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Q Hou et al. (2022), Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection Cell Reports, 40(13):111416.
PMID: 36170821
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

E Azzoni et al. (2021), The onset of circulation triggers a metabolic switch required for endothelial to hematopoietic transition Cell Reports, 37(11):110103.
PMID: 34910918
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

R Fu et al. (2020), Endothelial ZEB1 promotes angiogenesis-dependent bone formation and reverses osteoporosis Nat Commun, 11(1):460.
PMID: 31974363
Species: Rat
Sample Types: Whole Tissue
Applications: IHC

G Sharma et al. (2020), Heritable modifiers of the tumor microenvironment influence nanoparticle uptake, distribution and response to photothermal therapy Theranostics, 10(12):5368-5383.
PMID: 32373218
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

DH Yeom et al. (2020), ABL001, a Bispecific Antibody Targeting VEGF and DLL4, with Chemotherapy, Synergistically Inhibits Tumor Progression in Xenograft Models International Journal of Molecular Sciences, 22(1).
PMID: 33383646
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

C Viallard et al. (2020), BMP9 signaling promotes the normalization of tumor blood vessels Oncogene, 0(0).
PMID: 32042114
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Neal et al. (2019), Venous identity requires BMP signalling through ALK3 Nat Commun, 10(1):453.
PMID: 30692543
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-F

M Fernández- et al. (2019), iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications Nat Commun, 10(1):2262.
PMID: 31118412
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

D Yasuda et al. (2019), Lysophosphatidic acid-induced YAP/TAZ activation promotes developmental angiogenesis by repressing Notch ligand Dll4 J. Clin. Invest., 130(0).
PMID: 31335323
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

S Payne et al. (2019), Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 10(1):3276.
PMID: 31332177
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Z Gong et al. (2019), Fibrotic liver microenvironment promotes Dll4 and SDF-1-dependent T-cell lineage development Cell Death Dis, 10(6):440.
PMID: 31165736
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

F Tetzlaff et al. (2018), MPDZ promotes DLL4-induced Notch signaling during angiogenesis Elife, 7(0).
PMID: 29620522
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

AV Shah et al. (2017), The endothelial transcription factor ERG mediates Angiopoietin-1-dependent control of Notch signalling and vascular stability Nat Commun, 8(0):16002.
PMID: 28695891
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

RB Davis et al. (2017), Lymphatic deletion of calcitonin receptor-like receptor exacerbates intestinal inflammation JCI Insight, 2(6):e92465.
PMID: 28352669
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Toru Nakata et al. (2017), Data showing proliferation and differentiation of intestinal epithelial cells under targeted depletion of Notch ligands in mouse intestine. Data in Brief, 0(0):2352-3409.
PMID: 28124021
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Vijaykumar Chennupati (2016), Notch Signaling Regulates the Homeostasis of Tissue-Restricted Innate-like T Cells J Immunol, 0(0).
PMID: 27324132
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

(2016), Blood flow controls bone vascular function and osteogenesis Nat Commun, 7(0):13601.
PMID: 27922003
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Gopinathan G et al. (2015), Interleukin-6 Stimulates Defective Angiogenesis. Cancer Res, 75(15):3098-107.
PMID: 26081809
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Zhou Y et al. (2015), Sox7, Sox17, and Sox18 Cooperatively Regulate Vascular Development in the Mouse Retina. PLoS ONE, 10(12):e0143650.
PMID: 26630461
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Zou M et al. (2015), Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn, 244(1):86-97.
PMID: 25179941
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Rama N et al. (2015), Slit2 signaling through Robo1 and Robo2 is required for retinal neovascularization. Nat Med, 21(5):483-91.
PMID: 25894826
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Ramasamy S et al. (2014), Endothelial Notch activity promotes angiogenesis and osteogenesis in bone. Nature, 507(7492):376-80.
PMID: 24647000
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Samarelli A et al. (2014), Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin. J Biol Chem, 289(28):19466-76.
PMID: 24860089
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Izumi N et al. (2012), Fbxw7 controls angiogenesis by regulating endothelial notch activity. PLoS ONE, 7(7):e41116.
PMID: 22848434
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Benedito R et al. (2012), Notch-dependent VEGFR3 upregulation allows angiogenesis without VEGF-VEGFR2 signalling. Nature, 484(7392):110-4.
PMID: 22426001
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Stamataki D et al. (2011), Delta1 expression, cell cycle exit, and commitment to a specific secretory fate coincide within a few hours in the mouse intestinal stem cell system. PLoS ONE, 6(9):e24484.
PMID: 21915337
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Haller BK et al. (2010), Therapeutic efficacy of a DNA vaccine targeting the endothelial tip cell antigen delta-like ligand 4 in mammary carcinoma. Oncogene, 29(30):4276-86.
PMID: 20498640
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

Tammela T et al. (2008), Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation. Nature, 454(7204):656-60.
PMID: 18594512

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Mouse DLL4 Antibody

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Applications for Mouse DLL4 Antibody

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Western Blot

Immunocytochemistry

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Formulation, Preparation and Storage

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Background: DLL4

Citations for Mouse DLL4 Antibody (29)

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