Mouse CD8 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11723
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG Clone # 1104518
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived mouse CD8
Lys28-Tyr196
Accession # P01731
Lys28-Tyr196
Accession # P01731
Specificity
Detects recombinant mouse CD8alpha protein in Direct ELISA.
Clonality
Monoclonal
Host
Rat
Isotype
IgG
Scientific Data Images for Mouse CD8 alpha Antibody
Detection of Mouse CD8 alpha by Western Blot.
Western Blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11723) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD8 alpha at approximately 30 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Mouse CD8 alpha Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Mouse thymus tissue
Sample: Mouse thymus tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD8
CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha/Ly-2 or alpha′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).
References
- Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- Hayakawa, K. et al. (1994) Science 263:1131.
- MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
- Rocha, B. et al. (1992) Immunol. Today 13:449.
- Wang, J. and J.R. Klein (1994) Science 265:1860.
- Vermec, D. et al. (1992) J. Exp. Med. 176:47.
- Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Alternate Names
CD8, CD8A
Entrez Gene IDs
Gene Symbol
CD8A
UniProt
Additional CD8 Products
Product Documents for Mouse CD8 alpha Antibody
Product Specific Notices for Mouse CD8 alpha Antibody
For research use only
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