Mouse CD8 alpha Antibody

Name: Mouse CD8 alpha Antibody
Brand: R&D Systems
SKU: MAB11715

R&D Systems, part of Bio-Techne | Catalog # MAB11715

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Product Datasheet / COA / SDS

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line, NS0-derived mouse CD8 Lys28-Tyr196 Accession # P01731
Applications:	Immunohistochemistry, Multiplex Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 1104516
Specificity:	Detects recombinant mouse CD8 alpha protein in Direct ELISA.

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Technical Data
Citations / Reviews

Applications for Mouse CD8 alpha Antibody

	Recommended Concentration	Sample
Immunohistochemistry	3-25 µg/mL	Perfusion fixed paraffin-embedded sections of mouse spleen and thymus
Multiplex Immunofluorescence	5 µg/mL	Perfusion fixed paraffin-embedded sections of mouse spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD8 alpha Antibody

	Multiplex Immunofluorescence Detection of CD8a in Mouse spleen via seqIF™ staining on COMET™ CD8a was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD8a, Monoclonal Antibody (Catalog #MAB11715) at 5ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.
	Immunohistochemistry Detection of CD8 alpha in Mouse Spleen. CD8 alpha was detected in perfusion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11715) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry Detection of CD8 alpha in Mouse Thymus. CD8 alpha was detected in perfusion fixed paraffin-embedded sections of mouse thymus using Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11715) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma δ TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

Item	Value
References	Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280. Hayakawa, K. et al. (1994) Science 263:1131. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927. Rocha, B. et al. (1992) Immunol. Today 13:449. Wang, J. and J.R. Klein (1994) Science 265:1860. Vermec, D. et al. (1992) J. Exp. Med. 176:47. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.
Entrez Gene IDs	925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)
Alternate Names	CD8, CD8 antigen, alpha polypeptide (p32), CD8A, CD8a molecule, CD_antigen: CD8a, LEU2, Leu2 T-lymphocyte antigen, MAL, OKT8 T-cell antigen, T cell co-receptor, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, T8 T-cell antigen, p32

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Mouse CD8 alpha Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11715

Key Product Details

Applications for Mouse CD8 alpha Antibody

Scientific Data Examples for Mouse CD8 alpha Antibody

Multiplex Immunofluorescence

Immunohistochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: CD8

Customer Reviews for Mouse CD8 alpha Antibody

Product Documents for Mouse CD8 alpha Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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