Mouse CD5 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB115A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Rat IgG2A Clone # 53-7.3
Product Specifications
Immunogen
Mouse thymus or spleen
Specificity
Detects mouse CD5 in flow cytometry.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse CD5 APC-conjugated Antibody
Detection of CD5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse CD5 APC-conjugated Monoclonal Antibody (Catalog # FAB115A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse CD5 APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD5
Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.
References
- Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.
- Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
- van Ewijk, W. et al. (1981) J. Immunol. 127:2594.
- Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.
- Luo, W. et al. (1992) J. Immunol. 148:1630.
- Lanier, L.L. et al. (1986) J. Immunol. 137:2735.
Alternate Names
CD5
Gene Symbol
CD5
Additional CD5 Products
Product Documents for Mouse CD5 APC-conjugated Antibody
Product Specific Notices for Mouse CD5 APC-conjugated Antibody
For research use only
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