Mouse CD45 Antibody

Name: Mouse CD45 Antibody
Brand: Bio-Techne
Price: 135 USD
Availability: InStock

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF114

Citations (30)

(1)

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Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse CD45 Gln24-Lys425 Accession # NP_035340
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse CD45 in direct ELISAs and Western blots.

View Full Technical Details for Mouse CD45 Antibody

Applications for Mouse CD45 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse CD45 (Catalog # 114-CD)
Flow Cytometry	2.5 µg/10⁶ cells	Mouse splenocytes
Immunohistochemistry	3-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD45 Antibody

	Immunocytochemistry CD45 in RAW 264.7 Mouse Cell Line. CD45 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry CD45 in Mouse Liver. CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD45

Mouse CD45 (also known as Ly5 and leukocyte common antigen) is a 180‑220 kDa variably glycosylated member of the class 1 subtype of the protein tyrosine phosphatase family. It is synthesized as a 1291 amino acid (aa) precursor that contains a 23 aa signal sequence, a 541 aa extracellular domain (ECD), a 22 aa transmembrane segment, and a 705 aa cytoplasmic region. The ECD is coded for by exons 4‑16 of the CD45 gene. Alternate splicing of exon 4 (or A) (aa 30‑74), exon 5 (or B) (aa 75‑123) and exon 6 (or C) (aa 124‑169) define different lymphocyte populations and functional stages. Naïve T cells express exon 5 (CD45 RB), while activated T cells express neither exon 4, 5 or 6 (CD 45 RO). B cells express CD45 RABC, while resting NK cells express CD45 RA. Mouse CD45 ECD shares 60% and 44% aa sequence identity with rat and human full‑length CD45 ECD, respectively.

Item	Value
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names	B220, CD45, CD45 antigen, CD45R, CD_antigen: CD45, EC 3.1.3.48, EC:3.1.3.48, GP180, L-CA, LCA, LY5, Leukocyte common antigen, PTPRC, T200, T200 Glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C

Citations for Mouse CD45 Antibody (30)

Showing 1 - 10 of 30 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD45 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

K Xu et al. (2022), The protective effects of systemic dexamethasone on sensory epithelial damage and hearing loss in targeted Cx26-null mice Cell Death Disease, 2022;13(6):545.
PMID: 35688810
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

G Huang et al. (2022), Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition Theranostics, 12(9):4415-4430.
PMID: 35673580
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Xia et al. (2022), Chronic suppurative otitis media causes macrophage-associated sensorineural hearing loss Journal of Neuroinflammation, 19(1):224.
PMID: 36096817
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

RM Lasagni Vi et al. (2021), Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade Investigative Ophthalmology Visual Science, 2021;62(3):26.
PMID: 33729475
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

SS Qin et al. (2021), Intertumoral Genetic Heterogeneity Generates Distinct Tumor Microenvironments in a Novel Murine Synchronous Melanoma Model Cancers, 13(10).
PMID: 34064795
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

J Filtjens et al. (2021), Nociceptive sensory neurons promote CD8 T cell responses to HSV-1 infection Nature Communications, 12(1):2936.
PMID: 34006861
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

J Wang et al. (2021), A transcriptome atlas of the mouse iris at single-cell resolution defines cell types and the genomic response to pupil dilation Elife, 10(0).
PMID: 34783308
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

A Glasow et al. (2021), Dose and Dose Rate-Dependent Effects of Low-Dose Irradiation on Inflammatory Parameters in ApoE-Deficient and Wild Type Mice Cells, 10(11).
PMID: 34831473
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

SJ Jeong et al. (2020), Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 0(0).
PMID: 32929220
MT Massengill et al. (2020), Sectoral activation of glia in an inducible mouse model of autosomal dominant retinitis pigmentosa Sci Rep, 10(1):16967.
PMID: 33046772
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

V Rai et al. (2020), The immune response after noise damage in the cochlea is characterized by a heterogeneous mix of adaptive and innate immune cells Scientific Reports, 10(1):15167.
PMID: 32938973
Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC

AE Engler et al. (2020), Airway-Associated Macrophages in Homeostasis and Repair Cell Reports, 33(13):108553.
PMID: 33378665
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

A Shahapal et al. (2019), FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice Front Neurosci, 13(0):917.
PMID: 31543758
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S Schwager et al. (2018), Antibody-mediated delivery of VEGF-C potently reduces chronic skin inflammation JCI Insight, 3(23).
PMID: 30518687
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

LJ Yockey et al. (2018), Type I interferons instigate fetal demise after Zika virus infection Sci Immunol, 3(19).
PMID: 29305462
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

C Maranto et al. (2018), STAT5A/B BLOCKADE SENSITIZES PROSTATE CANCER TO RADIATION THROUGH INHIBITION OF RAD51 AND DNA REPAIR Clin. Cancer Res., 0(0).
PMID: 29483142
Species: Human
Sample Types: Whole Cells
Applications: ICC

C Carmona-Fo et al. (2017), Metabolic origins of spatial organization in the tumor microenvironment Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28246332
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

J Liu et al. (2017), Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion JCI Insight, 2(18).
PMID: 28931758
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

MX Hernandez et al. (2017), Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss Mol Neurodegener, 12(1):66.
PMID: 28923083
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Z Zuo et al. (2017), Immunization with Bacillus Calmette-Guï¿½rin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain Neurobiol. Dis, 101(0):27-39.
PMID: 28189498
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Jong-Gil Park (2016), CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis Sci Rep, 6(0):27938.
PMID: 27301791
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Mathias D et al. (2015), Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. PLoS ONE, 10(3):e0119661.
PMID: 25799423
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Liakhovitskaia A et al. (2014), Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this. Development, 141(17):3319-23.
PMID: 25139854
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Gobbetti T et al. (2012), Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine. Am. J. Pathol., 180(1):141-52.
PMID: 22067907
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2012), Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia. J Neuroinflammation, 9(0):174.
PMID: 22799573
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Arora D et al. (2011), Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling. J. Biol. Chem., 286(13):10918-29.
PMID: 21262971
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Chou S et al. (2010), Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A., 107(17):7799-804.
PMID: 20385801
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P

Graham BB et al. (2010), Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling. Am. J. Pathol., 177(3):1549-61.
PMID: 20671265
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Tigges U et al. (2008), FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages. Development, 135(3):523-32.
PMID: 18171688
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Kraman M et al. (0), Suppression of antitumor immunity by stromal cells expressing fibroblast activation protein-alpha. Scienc330(6005):827-30.
PMID: 21051638

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Mouse CD45 Antibody

Name: Anonymous

Application: IHC

Sample Tested: retinal faltmounts

Species: mice

Verified Customer | Posted 11/08/2022

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Mouse CD45 Antibody

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Immunocytochemistry

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Formulation, Preparation and Storage

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Background: CD45

Citations for Mouse CD45 Antibody (30)

Customer Reviews for Mouse CD45 Antibody (1)

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Mouse CD45 Antibody

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