Mouse CD45 Antibody

Name: Mouse CD45 Antibody
Brand: R&D Systems
SKU: MAB114

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB114

Citations (9)

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Species Reactivity:	Mouse
Immunogen:	Mouse thymus and spleen cells
Applications:	CyTOF-reported, Flow Cytometry, Functional Assay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2B Clone # 30-F11
Specificity:	Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).

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Applications for Mouse CD45 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Mouse splenocytes
Immunohistochemistry	5-25 µg/mL	Immersion fixed paraffin-embedded sections of mouse spleen
Immunoprecipitation	Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
Complement-dependent Cytotoxicity	Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
CyTOF-reported	Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	3-25 µg/mL	Immersion fixed mouse splenocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse CD45 Antibody

	Immunocytochemistry CD45 in Mouse Splenocytes. CD45 was detected in immersion fixed mouse splenocytes (positive staining) and Neuro‑2A mouse neuroblastoma cell line (negative staining) using Rat Anti-Mouse CD45 Monoclonal Antibody (Catalog # MAB114) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry CD45 in Mouse Spleen. CD45 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rat Anti-Mouse CD45 Monoclonal Antibody (Catalog # MAB114) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CD45

CD45, previously called LCA (leukocyte common antigen), T200, or Ly5 in mouse, is member C of the class 1 (receptor-like) protein tyrosine phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180‑220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1‑3). CD45 has several isoforms, expressed according to cell type, developmental stage and antigenic exposure (1‑5). The longest form, CD45RABC (called B220 in mouse), is expressed on B lymphocytes (5). The mouse CD45RABC cDNA encodes 1291 amino acids (aa), including a 23 aa signal sequence, a 541 aa extracellular domain containing the splicing region, a cysteine-rich region and two fibronectin type III domains, a 22 aa transmembrane sequence, and a 705 aa cytoplasmic domain that contains two phosphatase domains, D1 and D2. Only D1 has phosphatase activity. CD45R0 is the shortest form, lacking exons 4, 5 and 6 which encode
aa 30‑169. It is expressed on memory cells, while intermediate sizes are expressed on other T cells (3, 4, 6). CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds (3, 6‑8). CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton (9, 10). Galectin interaction can be fine-tuned by varying usage of the heavily O-glycosylated spliced regions and sialylation of N-linked carbohydrates (4, 9). Within the IS, CD45 dephosphorylates and negatively regulates the src family kinase, LCK (8‑10). In other leukocytes, CD45 influences differentiation and links immunoreceptor signaling with cytokine secretion and cell survival, partially overlapping in function with DEP-1/CD148 (11‑14). CD45 deletion causes in severe immunodeficiency, while point mutations may be associated with autoimmune disorders (6, 7).

Item	Value
References	Anderson, J.N. et al. (2004) FASEB J. 18:8. Streuli, M. et al. (1987) J. Exp. Med. 166:1548. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107. Earl, L.A. and L.G. Baum (2008) Immunol. Cell Biol. 86:608. Ralph, S.J. et al. (1987) EMBO J. 6:1251. Falahti, R. and D. Leitenberg (2008) J. Immunol. 181:6082. Tchilian, E.Z. and P.C.L. Beverley (2006) Trends Immunol. 27:146. McNiell, L. et al. (2007) Immunity 27:425. Chen, I-J. et al. (2007) J. Biol. Chem. 282:35361. Freiberg, B.A. et al. (2002) Nat. Immunol. 3:911. Zhu, J.W. et al. (2008) Immunity 28:183. Huntington, N.D. et al. (2006) Nat. Immunol. 7:190. Hesslein, D.G. et al. (2006) Proc. Natl. Acad. Sci. USA 103:7012. Cross, J.L. et al. (2008) J. Immunol. 180:8020. Ledbetter, J.A. and L.A. Herzenberg (1979) Immunol. Rev. 47:63.
Long Name	Cluster of Differentiation 45
Entrez Gene IDs	5788 (Human); 19264 (Mouse); 490255 (Canine); 100061950 (Equine)
Alternate Names	B220, CD45, CD45 antigen, CD45R, CD_antigen: CD45, EC 3.1.3.48, EC:3.1.3.48, GP180, L-CA, LCA, LY5, Leukocyte common antigen, PTPRC, T200, T200 Glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C

Citations for Mouse CD45 Antibody (9)

Showing 1 - 9 of 9 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse CD45 Antibody include:

Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Y Akazawa et al. (2022), Rubicon-regulated beta-1 adrenergic receptor recycling protects the heart from pressure overload Scientific Reports, 12(1):41.
PMID: 34996972
Species: Human
Sample Types: Whole Tissue
Applications: IHC

SJ Jeong et al. (2020), Deficiency of peroxiredoxin 2 exacerbates angiotensin II-induced abdominal aortic aneurysm Exp. Mol. Med., 0(0).
PMID: 32929220
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

L Elia et al. (2018), UHRF1 epigenetically orchestrates smooth muscle cell plasticity in arterial disease J. Clin. Invest., 0(0).
PMID: 29558369
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

EN Komegae et al. (2017), Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis PLoS ONE, 12(2):e0171796.
PMID: 28235052
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

KR Wiehagen et al. (2017), Combination of CD40 agonism and CSF-1R blockade reconditions tumor-associated macrophages and drives potent antitumor immunity Cancer Immunol Res, 0(0).
PMID: 29097420
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

F Bignami et al. (2017), Growth inhibition of formed corneal neovascularization following Fosaprepitant treatment Acta Ophthalmol, 0(0).
PMID: 28205389
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Kanapathippillai P et al. (2013), Nucleosomes contribute to increase mesangial cell chemokine expression during the development of lupus nephritis. Cytokine, 62(2):244-52.
PMID: 23561928
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr

(2012), Extracellular adenosine signaling induces CX3CL1 expression in the brain to promote experimental autoimmune encephalomyelitis. J Neuroinflammation, 9(0):193.
PMID: 22883932
Species: Porcine
Sample Types: Tissue Homogenates
Applications: Western Blot

Carter AJ et al. (2004), Long-term effects of polymer-based, slow-release, sirolimus-eluting stents in a porcine coronary model. Cardiovasc. Res., 63(4):617-24.
PMID: 15306217

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Mouse CD45 Antibody

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Immunocytochemistry

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Background: CD45

Citations for Mouse CD45 Antibody (9)

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