Detects mouse CD39L3/ENTPD3 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross‑reactivity with recombinant human CD39L3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse CD39L3/ENTPD3 Antibody

Detection of Mouse CD39L3/ENTPD3 by Simple Western^TM.

Simple Western lane view shows lysates of Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for CD39L3/ENTPD3 at approximately 127 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4464) . This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Detection of Mouse CD39L3/ENTPD3 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, mouse salivary gland, and mouse spinal cord. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse CD39L3/ENTPD3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4464) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD39L3/ENTPD3 at approximately 75-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse CD39L3/ENTPD3 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse CD39L3/ENTPD3 (Catalog # 4464-EN), see our available Western blot detection antibodies

Simple Western

20 µg/mL
Sample: Neuro-2A mouse neuroblastoma cell line

Western Blot

0.5 µg/mL
Sample: Neuro-2A mouse neuroblastoma cell line, mouse salivary gland, and mouse spinal cord.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD39L3/ENTPD3

CD39L3 (CD39-like 3; also HB6 and ectonucleoside triphosphate diphosphohydrolase-3/eNTPDase 3) is an 80 kDa, class I member of the GDA1/CD39 NTPase family of transmembrane (TM) enzymes. It is a cell surface glycoprotein that hydrolyses both extracellular ATP and ADP. Thus, it controls ATP activation of P2X ion channel purinoreceptors. Mouse CD39L3 is 529 amino acids (aa) in length. It contains two TM segments plus an N- and C-terminal cytoplasmic region. Based on human CD39L3, the mouse extracellular domain (ECD) will contain aa 44-485. Within the ECD, mouse CD39L3 shares 82% and 94% aa identity with human and rat CD39L3 ECD, respectively.

Long Name

Ecto-Nucleoside Triphosphate Diphosphohydrolase 3

Alternate Names

ATPDase, CD39L3, ENTPD3, HB6, NTPDase-3

Entrez Gene IDs

956 (Human); 215446 (Mouse); 316077 (Rat)

Gene Symbol

ENTPD3

UniProt

AAH79871

Additional CD39L3/ENTPD3 Products

Product Documents for Mouse CD39L3/ENTPD3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD39L3/ENTPD3 Antibody

For research use only

Citations
Reviews

Mouse CD39L3/ENTPD3 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4464

Key Product Details

Species Reactivity

Validated:

Cited:

Applications