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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 324205

Product Summary for Mouse CD36/SR-B3 Alexa Fluor® 647-conjugated Antibody

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse CD36/SR-B3
Gly30-Lys439
Accession # Q08857

Specificity

Detects mouse CD36/SR-B3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CD36 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse CD36/SR-B3 Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: J774A.1 mouse reticulum cell sarcoma macrophage cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD36/SR-B3

CD36 (alternatively known as platelet membrane glycoprotein IV (GPIV), thrombospondin receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3)) is an 88 kDa, integral membrane glycoprotein that belongs to the class B scavenger receptor family (1, 2). The molecule is described as being ditopic, with two transmembrane segments connected by an extracellular loop (3). Mouse CD36 is synthesized as a 472 amino acid (aa) protein that contains a 6 aa N‑terminal cytoplasmic domain, a 22 aa N‑terminal transmembrane segment, a 420 aa extracellular “loop”, a 22 aa C‑terminal transmembrane segment, and a 9 aa C‑terminal cytoplasmic tail (4). Both cytoplasmic tails are palmitoylated, with the C‑terminal tail involved in oxidized LDL binding (5, 6). With respect to the extracellular loop, the N‑terminal region is believed to bind both thrombospondin-1 and Plasmodium-infected erythrocytes. Other ligands for CD36 include long-chain fatty acids, collagen, phospholipids and apoptotic cells (1). The extracellular loop of mouse CD36 shares 94%, 92%, 84% and 84% aa sequence identity with the extracellular loops of rat, hamster, human and bovine CD36, respectively. Cells known to express CD36 include capillary endothelium, adipocytes, skeletal muscle cells, intestinal epithelium, smooth muscle cells and hematopoietic cells such as RBC’s, platelets and monocytes (1). On the surface of cells, CD36 is suggested to exist as a dimer in response to ligation (7). CD36 is reported to regulate fatty uptake, act as an angiogenic with TSP-1, and participate in the clearance of apoptotic phagocytes (1, 8).

References

  1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:795. 
  2. Silverstein, R.L. and M. Febbraio (2000) Curr. Opin. Lipid. 11:483. 
  3. Gruarin, P. et al. (2000) Biochem. Biophys. Res. Commun. 275:446. 
  4. Endemann, G. et al. (1993) J. Biol. Chem. 268:11811. 
  5. Malaud, E. et al. (2002) Biochem. J. 364:507.
  6. Tao, N. et al. (1996) J. Biol. Chem. 271:22315.
  7. Daviet, L. et al. (1997) Thromb. Haemost. 78:897.
  8. Simantov, R. and R.L. Silverstein (2003) Front. Biosci. 8:s874.

Long Name

Scavenger Receptor Class B, Member 3

Alternate Names

CD36, Collagen R, FAT, GPIIIb, GPIV, SCARB3, SR-B3, SRB3, Thrombospondin R

Entrez Gene IDs

948 (Human); 12491 (Mouse)

Gene Symbol

CD36

UniProt

Product Documents for Mouse CD36/SR-B3 Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD36/SR-B3 Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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