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Mouse CD3 Antibody
Catalog # MAB4841R | R&D Systems, Inc. a Bio-Techne Brand
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rat IgG Clone # 17A2R
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Product Summary for Mouse CD3 Antibody
Immunogen
T cell hybridoma D1
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG
Scientific Data Images for Mouse CD3 Antibody
Detection of CD3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with either Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB8481R, filled histogram) or Rat IgG2B Isotype Control (Catalog # MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.
CD3 in Mouse Splenocytes.
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
CD3 in Mouse Spleen Tissue.
CD3 was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse CD3 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunohistochemistry
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4841R in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3
References
- Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Alternate Names
CD3, CD3E, CD_antigen: CD3e, CD3 antigen, delta subunit, CD3-DELTA, CD3-epsilon, CD3-GAMMA, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3G, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T3DOKT3, delta chain, T3E, TCRE
Gene Symbol
CD3E
Product Documents for Mouse CD3 Antibody
Product Specific Notices for Mouse CD3 Antibody
For research use only
Customer Reviews for Mouse CD3 Antibody (1)
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: SplenocytesSpecies: MouseVerified Customer | Posted 10/22/2022
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FAQs for Mouse CD3 Antibody
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
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