Mouse CD3 Antibody
Catalog # MAB4841R | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
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Antibody Source
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Product Summary for Mouse CD3 Antibody
Immunogen
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse CD3 Antibody
Detection of CD3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with either Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB8481R, filled histogram) or Rat IgG2B Isotype Control (Catalog # MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.CD3 in Mouse Splenocytes.
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.CD3 in Mouse Spleen Tissue.
CD3 was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841R) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Mouse CD3 Antibody
CyTOF-ready
Flow Cytometry
Sample: Mouse splenocytes
Immunocytochemistry
Sample: Immersion fixed mouse splenocytes
Immunohistochemistry
Sample: Immersion fixed paraffin-embedded sections of mouse spleen tissue
Reviewed Applications
Read 1 review rated 5 using MAB4841R in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3
References
- Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Alternate Names
Gene Symbol
Product Documents for Mouse CD3 Antibody
Product Specific Notices for Mouse CD3 Antibody
For research use only
Customer Reviews for Mouse CD3 Antibody (1)
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: SplenocytesSpecies: MouseVerified Customer | Posted 10/22/2022
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FAQs for Mouse CD3 Antibody
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Q: What is the optimal working concentration of the antibody?
A: Novus' product datasheets provide a recommended concentration for each validated application that is a starting point for titration. The concentration required will vary from lab to lab due to other variables and thus titration is strongly recommended for optimizing results.
Reconstitution Calculator
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.