Detects mouse CD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human CD2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD2 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Mouse CD3⁺ splenocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

Mouse CD2, also known as LFA-2 and LFA-3 Receptor, is a 55‑66 kDa, variably glycosylated type I transmembrane (TM) protein belonging to the CD2 receptor subset of the Ig superfamily. It is synthesized as a 344 amino acid (aa) precursor that contains a 22 aa signal sequence, a 181 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a proline rich (20%) 115 aa cytoplasmic region. CD2 is found on T and NK cells and binds CD48 in rodents and CD58 in humans. The ECD of mouse CD2 shares 73%, 50% and 44% aa identity with the CD2 ECD in rat, human and equine, respectively.

Alternate Names

CD2, LFA-2, T11

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Gene Symbol

CD2

UniProt

P08920

Additional CD2 Products

Product Documents for Mouse CD2 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CD2 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Mouse CD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2828

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CD2 Antibodies »

Product Specifications

Immunogen

Specificity