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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Alexa Fluor 750 (Excitation = 749 nm, Emission = 775 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 612722

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived recombinant mouse CD163
Val39-Thr1045
Accession # Q2VLH6

Specificity

Detects mouse CD163 in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Applications for Mouse CD163 Alexa Fluor® 750-conjugated Antibody

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CD163

CD163, also known as M130 and  p155, is a 130-160 kDa type I transmembrane protein belonging to group B of the cysteine-rich scavenger receptor family (1). Mature mouse CD163 consists of a 1007 amino acid (aa) extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 21 aa transmembrane segment, and a 55 aa cytoplasmic domain (2). Alternative splicing of mouse CD163 generates an isoform with a substitution in the cytoplasmic region. Within the ECD, mouse CD163 shares 75% and 90% aa sequence identity with human and rat CD163, respectively. CD163 is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10 (3-5). A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage (6, 7) in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6 (8-10). CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines (11). It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin (12, 13). It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion (14). CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis (15, 16).

Alternate Names

CD163, GHI/61, HbSR, M130, RM3/1

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Gene Symbol

CD163

UniProt

Additional CD163 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD163 Alexa Fluor® 750-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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