Mouse CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1124
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-reported
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # M1/70
Product Specifications
Immunogen
Con A-activated C57BL/10 splenocytes
Specificity
Detects mouse CD11b/Integrin alphaM. Cross-reaction with human CD11b/Integrin alphaM has been reported (1, 2).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD11b/Integrin alpha M Antibody
Detection of CD11b/Integrin alpha M in Mouse Bone Marrow by Flow Cytometry
Mouse Bone Marrow Cells were stained with Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1124) or (B) isotype control antibody (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
CD11b/Integrin alphaM in Mouse Splenocytes.
CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse CD11b/Integrin alpha M Antibody
Application
Recommended Usage
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunoprecipitation
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alphaM subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta2 subunit (CD18) to form the non-covalent heterodimer Integrin alphaM/ beta2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alphaM/ beta2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alphaM/ beta2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
References
- Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
- Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for Mouse CD11b/Integrin alpha M Antibody
Product Specific Notices for Mouse CD11b/Integrin alpha M Antibody
For research use only
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