Mouse CD11b/Integrin alpha M Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1124
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry, Immunoprecipitation, CyTOF-reported
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # M1/70
Product Specifications
Immunogen
Con A-activated C57BL/10 splenocytes
Specificity
Detects mouse CD11b/Integrin alphaM. Cross-reaction with human CD11b/Integrin alphaM has been reported (1, 2).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse CD11b/Integrin alpha M Antibody
Detection of CD11b/Integrin alpha M in Mouse Bone Marrow by Flow Cytometry
Mouse Bone Marrow Cells were stained with Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) PE-conjugated Monoclonal Antibody (Catalog # FAB1037P) and either (A) Rat Anti-Mouse CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1124) or (B) isotype control antibody (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.
CD11b/Integrin alphaM in Mouse Splenocytes.
CD11b/Integrin aM was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB1124) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse CD11b/Integrin alpha M Antibody
Application
Recommended Usage
CyTOF-reported
Lee, H. et al. (2015) Mucosal Immunol. 8: 1083. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Mouse splenocytes
Sample: Mouse splenocytes
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Immunoprecipitation
Springer, T.A. et al. (1979) Eur. J. Immunol. 9:301.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD11b/Integrin alpha M
The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alphaM subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta2 subunit (CD18) to form the non-covalent heterodimer Integrin alphaM/ beta2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alphaM/ beta2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alphaM/ beta2 can bind several ligands (including ICAM-1, fibrinogen, and the C3 complement fragment, C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.
References
- Beller, D.J. et al. (1982) J. Exp. Med. 156:1000.
- Ault, K.A. and T.A. Springer (1981) J. Immunol. 126:359.
Alternate Names
CD11b, Integrin alpha M, ITGAM
Gene Symbol
ITGAM
Additional CD11b/Integrin alpha M Products
Product Documents for Mouse CD11b/Integrin alpha M Antibody
Product Specific Notices for Mouse CD11b/Integrin alpha M Antibody
For research use only
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