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Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2B Clone # 225307

Product Summary for Mouse CCR5 Antibody

Immunogen

C6 rat glioma cell line transfected with mouse CCR5
Accession # P51682

Specificity

Detects mouse CCR5 transfectants but not the parental cell line in Flow Cytometry and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR5 Antibody

Detection of CCR5 antibody in HEK293 Human Cell Line Transfected with Mouse CCR5 and eGFP antibody by Flow Cytometry.

Detection of CCR5 in HEK293 Human Cell Line Transfected with Mouse CCR5 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with mouse CCR5 and eGFP was stained with (A) Rat Anti-Mouse CCR5 Monoclonal Antibody (Catalog # MAB18041) or (B) Rat IgG2B isotype control antibody (Catalog # MAB0061) followed by PE-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Mouse CCR5 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 (CC chemokine receptor 5; also CD195 and MIP-1a receptor) is a 41 - 44 kDa member of the G-protein coupled receptor #1 family of proteins.  It is expressed on Treg cells, NK cells, neurons, macrophages and Th1 cells. CCR5 mediates cell adhesion and migration induced by several chemokines including CCL3/MIP-1 alpha, CCL4/MIP-1 beta, CCL5/RANTES, and CCL8/MCP-2. It also functions as a coreceptor for macrophage-tropic HIV-1 infection. CCR5 contains an O-glycosylated and sulfated extracellular N-terminus (aa 1 - 32), and a phosphorylated and palmitoylated intracellular C-terminus (aa 304 - 354).  CCR5 will form homodimers, heterodimers with CCR2, and heterooligomers with CCR2 and CXCR4. Within aa 1 - 32, mouse CCR5 shares 72% and 91% amino acid sequence identity with human and rat CCR5, respectively.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

UniProt

Additional CCR5 Products

Product Documents for Mouse CCR5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCR5 Antibody

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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