Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Mouse CCR2 Antibody

Catalog # MAB55381 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
Availability
Size / Price
Qty
Loading...
MAB55381-100
MAB55381-SP

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 475301

Product Summary for Mouse CCR2 Antibody

Immunogen

L1.2 mouse pro-B cell line transfected with mouse CCR2

Specificity

Detects mouse CCR2 transfectants but not the parental cell line in flow cytometry. Does not detect HEK293 human embryonic kidney cell line transfected with mouse CCR5 in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse CCR2 Antibody

Detection of CCR2 antibody in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP antibody by Flow Cytometry.

Detection of CCR2 in NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with either (A) mouse CCR2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse/Rat CCR2 Monoclonal Antibody (Catalog # MAB55381) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). Quadrant markers were set based on isotype control (Rat IgG2B,Catalog # MAB0061; data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse CCR2 Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: NS0 Mouse Cell Line Transfected with Mouse CCR2 and eGFP
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB55381 in the following applications:

Published Applications

Read 16 publications using MAB55381 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
Loading...

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Mouse CCR2 shares 67% and 95% aa sequence identity with human and rat CCR2, respectively.

Alternate Names

CC-CKR-2, CCR2, CCR2B, CD192, CKR2, CKR2A, CMKBR2, FLJ78302, MCP-1-R

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Gene Symbol

CCR2

Additional CCR2 Products

Product Documents for Mouse CCR2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CCR2 Antibody

For research use only

Loading...
Loading...

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=
Loading...
Setting the standard in quality research reagents