Detects mouse MDC in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity recombinant human (rh) MDC, recombinant mouse (rm) 6Ckine, rmFractalkine, and rmTARC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse CCL22/MDC Biotinylated Antibody

Mouse CCL22 / MDC ELISA Standard Curve

Recombinant Mouse CCL22/MDC (Catalog # 439-MD) was serially diluted and captured by Rat Anti-Mouse CCL22/MDC Monoclonal Antibody (Catalog # MAB4391) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse CCL22/MDC Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CCL22/MDC (Catalog # 439-MD)

Mouse CCL22/MDC Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Mouse CCL22/MDC Antibody (Catalog # MAB4391)
Standard: Recombinant Mouse CCL22/MDC Protein (Catalog # 439-MD)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using BAF439 in the following applications:

ELISA (2 Reviews)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL22/MDC

Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.

References

Schaniel, C. et al. (1998) J. Exp. Med. 188:451.
Imai, T. et al. (1998) J. Biol. Chem. 273:1764.
Godiska, R. et al. (1997) J. Exp. Med. 185:1595.
Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

Alternate Names

ABCD-1, MDC, STCP-1

Entrez Gene IDs

6367 (Human); 20299 (Mouse)

Gene Symbol

CCL22

UniProt

Q9QZU2

Additional CCL22/MDC Products

Product Documents for Mouse CCL22/MDC Biotinylated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse CCL22/MDC Biotinylated Antibody

For research use only

Citations
Reviews

Mouse CCL22/MDC Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF439

Key Product Details

Species Reactivity

Validated:

Cited:

Applications