Detects mouse Cathepsin H in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin H. In Western blots, no cross-reactivity with rmCathepsin A, D, E, X, and Z or recombinant human Cathepsin B, C, L, or S is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse Cathepsin H Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Cathepsin H (Catalog # 1013-CY)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin H

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

References

Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego.
Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
Guncar, G. et al. (1998) Structure 6:51.
Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.

Alternate Names

CTSH

Entrez Gene IDs

1512 (Human); 13036 (Mouse)

Gene Symbol

CTSH

UniProt

Q3UCD6

Additional Cathepsin H Products

Product Documents for Mouse Cathepsin H Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse Cathepsin H Antibody

For research use only

Citations
Reviews

Mouse Cathepsin H Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1013

Key Product Details

Species Reactivity

Validated:

Cited:

Applications