BOC was detected in perfusion fixed frozen sections of mouse embryo using Goat Anti-Mouse BOC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2385) at 15 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of the neural tube, spinal cord. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse BOC Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse embryo (E13.5-15.5)

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse BOC

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BOC

BOC and CDO (cell adhesion molecule-related/down-regulated by oncogenes) are cell surface receptors belonging to the Ig/fibronectin type III (FNIII) repeat family. Both proteins have five Ig repeats and three FNIII repeats in their extracellular domains, an arrangement shared by Robo proteins. BOC and CDO are co-expressed in muscle precursor cells during development. BOC associates with CDO in cis and forms complexes with cadherins to regulate myogenic differentiation.

Long Name

Brother of CDO

Alternate Names

Boc homolog (mouse), brother of CDO, Protein BOC

Entrez Gene IDs

91653 (Human); 117606 (Mouse)

Gene Symbol

BOC

UniProt

AAH78631

Additional BOC Products

Product Documents for Mouse BOC Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Mouse BOC Antibody

For research use only

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