Detects mouse BMP‑15/GDF‑9B in direct ELISAs. In this format, no cross-reactivity with recombinant human BMP1.1, 2, 3, 3b, 4, 5, 6, 7, 8, 9, 10, 15, recombinant mouse BMP-3, 3b, 4, 5, 6, 7, 8b, 9, or 10 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Mouse BMP-15/GDF-9B Antibody

BMP‑15/GDF‑9B in Mouse Ovary.

BMP-15/GDF-9B was detected in perfusion fixed frozen sections of adult mouse ovary using Mouse BMP-15/GDF-9B Monoclonal Antibody (Catalog # MAB5917) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to an oocyte within the ovary. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse BMP-15/GDF-9B Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse ovary

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-15/GDF-9B

Bone morphogenetic protein 15 (BMP-15), also known as GDF-9B, is a member of the TGF-beta superfamily. Mature BMP-15 has three intrachain disulfide bands that form a cysteine-knot fold. It is secreted as a 34 kDa non-disulfide-linked homodimer and as a 37 kDa nondisulfide heterodimer with GDF-9. BMP-15 is a product of oocytes and promotes granulosa cell proliferation and stem cell factor secretion. Mature mouse BMP-15 shares 44% aa sequence identity with mature mouse GDF-9. It also shares 70% and 78% aa sequence identity with human and sheep BMP-15, respectively.

Long Name

Bone Morphogenetic Protein 15

Alternate Names

BMP15, GDF-9B

Entrez Gene IDs

9210 (Human); 12155 (Mouse); 59302 (Rat)

Gene Symbol

BMP15

UniProt

Q9Z0L4

Additional BMP-15/GDF-9B Products

Product Documents for Mouse BMP-15/GDF-9B Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse BMP-15/GDF-9B Antibody

For research use only

Citations
Reviews

Mouse BMP-15/GDF-9B Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5917

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all BMP-15/GDF-9B Antibodies »

Product Specifications

Immunogen

Specificity