Mouse B7-H2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF158
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Validated:
Western Blot, Immunocytochemistry
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Glu47-Lys279
Accession # Q9JHJ8
Glu47-Lys279
Accession # Q9JHJ8
Specificity
Detects mouse B7‑H2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse B7-H2 Antibody
B7-H2 in Mouse Splenocytes.
B7-H2 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse B7-H2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF158) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Mouse B7-H2 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed mouse splenocytes
Sample: Immersion fixed mouse splenocytes
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse B7-H2 Fc Chimera (Catalog # 158-B7)
Sample: Recombinant Mouse B7-H2 Fc Chimera (Catalog # 158-B7)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: B7-H2
Mouse B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. The extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20‑25% amino acid identity. Mouse and human B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Mouse B7-H2 is a 322 amino acid (aa) protein with a putative 46 aa signal peptide, a 233 aa extracellular domain, a 19 aa transmembrane region, and a 24 aa cytoplasmic domain. Mouse B7-H2 is expressed constitutively on resting B cells and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Ling, V. et al. (2000) J. Immunol. 164:1653.
- Wang, S. et al. (2000) Blood 96:2808.
- Brodie, D. et al. (2000) Curr. Biol. 10:333.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Swallow, M.M. et al. (1999) Immunity 11:423.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Mouse B7-H2 Antibody
Product Specific Notices for Mouse B7-H2 Antibody
For research use only
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