Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4886
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A
Val33-Ser362
Accession # AAH04572
Val33-Ser362
Accession # AAH04572
Specificity
Detects mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ASAHL is observed and less than 5% cross-reactivity rhASAHL-2 and recombinant mouse ASAHL-2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
Detection of Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL by Western Blot.
Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4886) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of ASAHL/N-acylethanolamine-hydrolyzing Acid A by Western Blot
Characterization of NAAA-/- mice. (A) Tail genomic analysis of WT and NAAA−/- mouse by PCR. (B) Representative western-blot bands of NAAA abundances in the spleen, lungs, brain, and skin from WT and NAAA-/- mice. (C) Representative immunohistochemical analyses of NAAA in the brain and lung sections from WT and NAAA−/- mice. (D) PEA hydrolytic activities were tested in the lysosomal fraction of brain, lungs and spleen homogenates from WT and NAAA−/- mice, at pH 4.5. Data are expressed as mean ± SEM (n = 5). *, p < 0.05; **, p < 0.01 by t test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35069223), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4886-AH), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL (Catalog # 4886-AH), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Mouse lung tissue
Sample: Mouse lung tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase
The mouse ASAHL gene encodes N-acylethanolamine-hydrolyzing Acid Amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-, N-stearoyl-, N-oleoyl-, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the choloylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (1). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (1). Through its amidase activity, ASAHL may play a role in the termination of the actions of a variety of N-acylethanolamides (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonoyl fluorophosphonate (2).
References
- Tsuboi, K. et al. (2005) J. Biol. Chem. 280:11082.
- Ueda, N. et al. (2001) J. Biol. Chem. 276:35552.
- Sun, Y. X. et al. (2005) Biochim. Biophys. Acta 1736:211.
Alternate Names
Acid ceramidase-like protein, ASAH-like protein, NAAA, Nacylethanolaminehydrolyzing Acid Amidase
Gene Symbol
NAAA
UniProt
Additional ASAHL/N-acylethanolamine-hydrolyzing Acid Amidase Products
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Product Documents for Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
Product Specific Notices for Mouse ASAHL/N-acylethanolamine-hydrolyzing Acid A Antibody
For research use only
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