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Mouse Aminopeptidase N/CD13 Antibody

Catalog # MAB2335 | R&D Systems, Inc. a Bio-Techne Brand
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MAB2335
MAB2335-SP

Key Product Details

Species Reactivity

Mouse

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 945033

Product Summary for Mouse Aminopeptidase N/CD13 Antibody

Immunogen

NS0 mouse myeloma cell line transfected with mouse Aminopeptidase
Lys69-Ser966
Accession # P97449

Specificity

Detects mouse Aminopeptidase in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Aminopeptidase N/CD13 Antibody

Detection of Mouse Aminopeptidase N/CD13 antibody by Western Blot.

Detection of Mouse Aminopeptidase N/CD13 by Western Blot.

Western blot shows lysates of mouse kidney tissue and mouse uterus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB2335) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Aminopeptidase N/CD13 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Mouse Aminopeptidase N/CD13 antibody by Simple WesternTM.

Detection of Mouse Aminopeptidase N/CD13 by Simple WesternTM.

Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Aminopeptidase N/CD13 at approximately 204 kDa (as indicated) using 10 µg/mL of Rat Anti-Mouse Aminopeptidase N/CD13 Monoclonal Antibody (Catalog # MAB2335) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse Aminopeptidase N/CD13 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Mouse kidney tissue

Western Blot

1 µg/mL
Sample: Mouse kidney tissue and mouse uterus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase N/CD13

The mouse Anpep gene encodes Aminopeptidase N (APN), which is also known as microsomal aminopeptiase, alanyl aminopeptidase, Aminopeptidase M, CD13, or membrane protein p161 (1‑3). The deduced amino acid sequence of mouse APN consists of a short cytoplasmic tail (residues 2 to 8), a transmembrane region (residue 9 to 32), a Ser/Thr rich region and a zinc metalloprotease domain (residues 69 to 966). Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. The roles of APN in many fields, such as neuroscience, hematopoeitic cells, immune system, angiogenesis, cancer and viral infection, have been reviewed (4).

References

  1. Chen, H. et al. (1996) J. Immunol. 157:2593.
  2. Larsen, S.L. et al. (1996) J. Exp. Med. 184:183.
  3. Hansen, A.S. et al. (1993) Eur. J. Immunol. 23:2358.
  4. Turner, A.J. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 289 Academic Press, San Diego.

Alternate Names

Aminopeptidase M, ANPEP, APN, CD13, gp150, PEPN

Entrez Gene IDs

290 (Human); 16790 (Mouse); 397520 (Porcine); 102115663 (Cynomolgus Monkey)

Gene Symbol

ANPEP

UniProt

Additional Aminopeptidase N/CD13 Products

Product Documents for Mouse Aminopeptidase N/CD13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Aminopeptidase N/CD13 Antibody

For research use only

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