Mouse 4-1BB Ligand/TNFSF9 Antibody

Name: Mouse 4-1BB Ligand/TNFSF9 Antibody
Brand: R&D Systems
SKU: AF1246

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1246

Citations (5)

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Species Reactivity:	Mouse
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse 4‑1BB Ligand/TNFSF9 Arg104-Glu309 Accession # P41274
Applications:	Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse 4‑1BB Ligand/TNFSF9 in direct ELISAs and Western blots. In Western blots, approximatley 5% cross‑reactivity with recombinant mouse (rm) Fas Ligand is observed and less than 1% cross-reactivity with rmOX40 Ligand, rmTWEAK, rmTRAIL, and rmTNF-alpha is observed.

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Applications for Mouse 4-1BB Ligand/TNFSF9 Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Mouse 4‑1BB Ligand/TNFSF9 (Catalog # 1246-4L)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: 4-1BB Ligand/TNFSF9

4-1BB ligand (4-1BBL) is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF9. Mouse 4-1BBL cDNA encodes a 309 amino acid residues (aa) protein with an 82 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 206 aa C-terminal extracellular domain. The extracellular domain of 4-1BBL has a tertiary structure similar to that of other TNFSF members, but shares only low aa sequence homology (14‑16%). Murine 4-1BBL shares 36% aa sequence identity with its human counterpart (1, 2). 4-1BBL is predominantly expressed on activated antigen presenting cells (APCs) such as B cells, macrophages and dendritic cells (DCs). It is also expressed on most T and B lymphoma cell lines (3). A soluble 4-1BBL is released from the cell surface following cellular activation via proteolytic cleavage by one or more sheddases (4). By analogy to other TNFSF ligands, both the soluble and transmembrane 4-1BBL are expected to exist as non-covalent homotrimers. 4-1BBL binds 4-1BB, a TNF receptor superfamily member, TNFRSF9, which is also known as CD137 and ILA (induced by lymphocyte activation). 4-1BB is expressed on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells. It is also expressed on monocytes, neutrophils, DCs and eosinophils. In response to 4-1BBL binding, 4-1BB transduces a T cell costimulatory signal in both CD4⁺ and CD8⁺ T cells to promote survival and enhance proliferation, cytokine production and effector function. In dendritic cells, 4-1BB is a DC-activating molecule that enhances cytokine production and up‑regulates expression of B7-1 and B7-2 costimulatory molecules (3).

Item	Value
References	Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631. Alderson, M.R. et al. (1994) Eur. J. Immunol. 24:2219. Kwon, B., H.W. Lee and B.S Kwon (2002) TRENDS in Immunology 23:378. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
Entrez Gene IDs	8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)
Alternate Names	4-1BB Ligand, 4-1BB-L, 4-1BBL, 41BB Ligand, CD137L, TNFSF9, homolog of mouse 4-1BB-L, receptor 4-1BB ligand, tumor necrosis factor (ligand) superfamily, member 9, tumor necrosis factor ligand superfamily member 9

Citations for Mouse 4-1BB Ligand/TNFSF9 Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Mouse 4-1BB Ligand/TNFSF9 Antibody include:

Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

V Laspidea et al. (2022), Exploiting 4-1BB immune checkpoint to enhance the efficacy of oncolytic virotherapy for diffuse intrinsic pontine gliomas JCI Insight, 7(7).
PMID: 35393952
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Eun S et al. (2015), 4-1BB ligand signaling to T cells limits T cell activation. J Immunol, 194(1):134-41.
PMID: 25404362
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Kanagavelu et al. (2014), HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity. PLoS ONE, 9(2):e90100.
PMID: 24587225
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Huang JH et al. (2010), Therapeutic and tumor-specific immunity induced by combination of dendritic cells and oncolytic adenovirus expressing IL-12 and 4-1BBL. Mol. Ther., 18(2):264-74.
PMID: 19738604
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Kang YJ et al. (2007), Cell surface 4-1BBL mediates sequential signaling pathways 'downstream' of TLR and is required for sustained TNF production in macrophages. Nat. Immunol., 8(6):601-9.
PMID: 17496895

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Showing 1 - 5 of 5 citations Showing All

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Mouse 4-1BB Ligand/TNFSF9 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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