MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29699

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Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 114-6G6

Format

Azide Free

Concentration

2 mg/ml

View all MMP-14/MT1-MMP Antibodies »

Product Specifications

Immunogen

This antibody is specific to MMP-14

Specificity

The antibody specifically reacts with human MT1-MMP. This is a purified mouse monoclonal antibody to an oligopeptide of REVPYAYIREGHEK (residue 160-173) on human membrane-type 1 matrix metalloproteinase (human MT1-MMP) and does not react with mouse or rat. However, this sequence is identical in mouse and rat MT1-MMP. For unknown reasons this antibody does not react on westerns to CC1043.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Relevant Entrez Gene Numbers: NM_004995.2

Scientific Data Images for MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Immunohistochemistry: MMP-14/MT1-MMP Antibody (114-6G6) [NBP2-29699]

Immunohistochemistry: MMP-14/MT1-MMP Antibody (114-6G6) [NBP2-29699]

Immunohistochemistry: MMP-14/MT1-MMP Antibody (114-6G6) [NBP2-29699] - Mouse anti-MT1-MMP Staining of breast carcinoma.

Applications for MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

8-20 ug/ml
Application Notes
ImmunoBlotting: 66+60kDa (Ueno et al., 1997) Immunohistochemistry on paraffin-embedded tissue sections, use at 20 ug/mL concentration (see Ueno et al., 1997; Nakamura et al., 1999). Requires periodate-lysine-paraformaldehyde fixative (Namura et al., 1999) for 18-24 hrs. at 4C. EIA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M Sodium Phosphate (pH 7.0) and 2% BSA (protease free)

Format

Azide Free

Preservative

No Preservative

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: MMP-14/MT1-MMP

The matrix metalloproteinase (MMP) family (approximately 25 members in mammals) has been implicated in extracellular matrix remodeling associated with embryonic development, cancer formation and progression, and various other physiological and pathological events. MMP-14 is a membrane-bound matrix metalloproteinase (MT-MMP) capable of mediating pericellular proteolysis of extracellular matrix components. MMP-14 is therefore thought to be an important molecular tool for cellular remodeling of the surrounding matrix (1). Gelatinase A (type-IV collagenase; M(r) 72,000) is produced by tumour stroma cells and is believed to be crucial for their invasion and metastasis, acting by degrading extracellular matrix macro-molecules such as type IV collagen. MMP-14 may thus trigger invasion by tumor cells by activating pro-gelatinase A on the tumor cell surface (2). Observations identify MT1-MMP as a tumor-derived growth factor that regulates proliferation by controlling cell geometry within the confines of the 3D extracellular matrix (ECM) (3).

Long Name

Matrix Metalloproteinase 14/Membrane Type 1 MMP

Alternate Names

MMP-X1, MMP14, MT-MMP1, MT1-MMP

Gene Symbol

MMP14

UniProt

P50281

Additional MMP-14/MT1-MMP Products

Product Documents for MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MMP-14/MT1-MMP Antibody (114-6G6) - Azide Free

Relevant Entrez Gene Numbers: NM_004995.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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