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mGluR8 Antibody - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NLS925

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NLS925

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bat, Equine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

0.79 mg/ml

Product Summary for mGluR8 Antibody - BSA Free

Immunogen

Synthetic 19 amino acid peptide from C-terminus of human mGluR8.

Epitope

C-Terminus

Predicted Species

Chimpanzee (100%), Opossum (95%). Backed by our 100% Guarantee.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Baboon (100%), Galago (100%), Marmoset (100%), Xenopus (89%).

Localization

Multi-pass membrane protein.

Specificity

Human mGluR8. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for mGluR8 Antibody - BSA Free

Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Anti-GRM8 / MGLUR8 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with brain, neurons.
Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with human brain, neurons and glia at 7 ug/ml

Applications for mGluR8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

7 - 18 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.79 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: mGluR8

Metabotropic Glutamate Receptor GRM8 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. Based on sequence homology, putative signal transduction mechanisms, and pharmacologic properties, GRM8 has been placed in Group III along with GRM4, GRM6, and GRM7. Group II and Group III receptors are linked to inhibition of the cyclic AMP signaling cascade, but differ in their agonist selectivities. Three isoforms of GRM8 are produced by alternative splicing. GRM8 has been reported primarily in the brain. ESTs have been isolated from B-cell/lung/testis, brain, breast, kidney, and testis libraries.

Long Name

Metabotropic Glutamate Receptor 8

Alternate Names

GPRC1H, GRM8

Entrez Gene IDs

2918 (Human)

Gene Symbol

GRM8

Additional mGluR8 Products

Product Documents for mGluR8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for mGluR8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Protocols

View specific protocols for mGluR8 Antibody - BSA Free (NLS925):

Immunohistochemistry Protocol for metabotropic Glutamate Receptor 8 Antibody (NLS925):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4-um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes at 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene approximately 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol approximately 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol approximately 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol approximately 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water approximately 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block approximately 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody approximately 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T approximately 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary approximately 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
17. Apply an alkaline phosphatase steptavidin approximately 30 minutes at RT.
18. Rinse the slide in 1X TBS-T approximately 1 minute at RT.
19. Apply an alkaline phosphatase chromagen substrate approximately 30 minutes at RT.
20. Rinse the slide in distilled water approximately 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol approximately 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol approximately 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol approximately 1 minute each @ RT.
24. Wash the slides in 3 changes of xyleneapproximately 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

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