Melan-A/MART-1 Antibody (A103) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46603
![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0002.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]")
![Western Blot: Melan-A/MART-1 Antibody (A103)BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-Western-Blot-NBP2-46603-img0005.jpg "Western Blot: Melan-A/MART-1 Antibody (A103)BSA Free [NBP2-46603]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # A103
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Recombinant human Melan-A/MART-1. [Uniprot# Q16655]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for Melan-A/MART-1 Antibody (A103) - BSA Free
Western Blot: Melan-A/MART-1 Antibody (A103)BSA Free [NBP2-46603]
Western Blot: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Total protein from human skin, pancreas and kidney and mouse kidney was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Melan-A in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.![Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-Immunohistochemistry-Paraffin-NBP2-46603-img0003.jpg "Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]")
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]
Immunohistochemistry-Paraffin: Melan-A/MART-1 Antibody (A103) [NBP2-46603] - Analysis of FFPE tissue section of Human Skin Cancer/Melanoma using Melan-A/MART-1 antibody (A103) at 1:100 dilution. The antibody depicted very specific staining which was cytoplasmic with strong punctate appearance in peri-nuclear regions (ER and Golgi) of most of the cancerous cells and membranous-cytoplasmic pattern in some necrotic areas.![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-BSA-Free-Flow-Cytometry-NBP2-46603-img0007.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]")
Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]
Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603] - An intracellular stain was performed on SK-MEL-28 cells with Melan-A/MART-1 Antibody (A103) NBP2-46603AF700 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 700.![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0004.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]")
![Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]](https://resources.bio-techne.com/images/products/Melan-A-MART-1-Antibody-A103-Flow-Cytometry-NBP2-46603-img0006.jpg "Flow Cytometry: Melan-A/MART-1 Antibody (A103) - BSA Free [NBP2-46603]")
Applications for Melan-A/MART-1 Antibody (A103) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 27565163)
Immunohistochemistry
1:100
Immunohistochemistry-Frozen
1:100
Immunohistochemistry-Paraffin
1:100
Western Blot
2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Melan-A/MART-1
Alternate Names
Antigen LB39-AA, Antigen SK29-AA, MART-1, MelanA, MLANA
Gene Symbol
MLANA
Additional Melan-A/MART-1 Products
Product Documents for Melan-A/MART-1 Antibody (A103) - BSA Free
Product Specific Notices for Melan-A/MART-1 Antibody (A103) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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