MED25 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-55868
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (94%), Rat (94%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: PPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGVPPPQASLHHLQPPG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit MED25 Antibody - BSA Free (NBP2-55868) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-MED25 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for MED25 Antibody - BSA Free
![Western Blot: MED25 Antibody [NBP2-55868]](https://resources.bio-techne.com/images/products/MED25-Antibody-Western-Blot-NBP2-55868-img0002.jpg "Western Blot: MED25 Antibody [NBP2-55868]")
Western Blot: MED25 Antibody [NBP2-55868]
Western Blot: MED25 Antibody [NBP2-55868] - Analysis in human cell line RT-4 and human cell line U-251 MG.![Immunocytochemistry/ Immunofluorescence: MED25 Antibody [NBP2-55868]](https://resources.bio-techne.com/images/products/MED25-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55868-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MED25 Antibody [NBP2-55868]")
Immunocytochemistry/ Immunofluorescence: MED25 Antibody [NBP2-55868]
Immunocytochemistry/Immunofluorescence: MED25 Antibody [NBP2-55868] - Staining of human cell line A549 shows localization to nucleoplasm. Antibody staining is shown in green.![MED25 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MED25 Antibody - BSA Free [NBP2-55868]](https://resources.bio-techne.com/images/products/nbp2-55868_rabbit-polyclonal-med25-antibody-25620259121962.jpg "Chromatin Immunoprecipitation-exo-Seq: MED25 Antibody - BSA Free [NBP2-55868]")
Chromatin Immunoprecipitation-exo-Seq: MED25 Antibody - BSA Free [NBP2-55868]
ChIP-Exo-Seq composite graph for Anti-MED25 (NBP2-55868) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Applications for MED25 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: MED25
Alternate Names
ACID1, Activator Interaction Domain-Containing Protein 1, Activator-Recruited Cofactor 92 KDa Component, ARC/Mediator Transcriptional Coactivator Subunit, ARC92, BVSYS, CMT2B2, Mediator Complex Subunit 25, Mediator Of RNA Polymerase II Transcription Subunit 25, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog, Mediator Of RNA Polymerase II Transcription, Subunit 25 Homolog (S. Cerevisiae), P78, TCBAP0758
Gene Symbol
MED25
Additional MED25 Products
Product Documents for MED25 Antibody - BSA Free
Product Specific Notices for MED25 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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