MED24 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74599
![Western Blot: MED24 Antibody [NB100-74599]](https://resources.bio-techne.com/images/products/MED24-Antibody-Western-Blot-NB100-74599-img0001.jpg "Western Blot: MED24 Antibody [NB100-74599]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Chicken (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 939 and 989 of human thyroid hormone receptor-associated protein complex 100 kDa component (mediator complex subunit 24 ) using the numbering given in entry NP_055630.2 (GeneID 986).
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MED24 Antibody - BSA Free
Western Blot: MED24 Antibody [NB100-74599]
Western Blot: MED24 Antibody [NB100-74599] - Detection of Human and Mouse TRAP100/MED24 on HeLa whole cell lysate using NB100-74599.
Western Blot: MED24 Antibody - BSA Free [NB100-74599] -
Most Mediator subunits are not altered in aging muscles. (A) Heat map of z-score–transformed expression of Mediator genes from RNA-seq of 10- and 30-months-old wild type (Wt) TA muscles. (B) RT-qPCR of Tail, (C) Middle, (D) Head, and (E) Kinase subunits of Mediator, and (F) myogenic genes in TA muscles from 3- and 24-months-old Wt mice. (G) Protein levels and relative quantification of representative subunits in TA muscles from 3- and 24- months-old Wt mice. N = 3/group, *p < 0.05 compared to 3 months by Welch’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38690566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Western Blot: MED24 Antibody - BSA Free [NB100-74599] -
Mediator subunit expression is decreased in differentiated C2C12 myotubes in vitro. (A) Heat map of z-score–transformed expression of Mediator genes from RNA-seq of C2C12 myoblasts (D0) and myotubes (D5) after 5 days of differentiation. (B) RT-qPCR of Tail, (C,D) Middle, (E,F) Head, and (G) Kinase subunits in C2C12 myoblasts (D0) and myotubes after 1, 3, and 5 days of differentiation (D1, D3, D5). (H) Protein levels and relative quantification of representative subunits in C2C12 cells before and after differentiation. N = 3/group, *p < 0.05 compared to D0 by one-way ANOVA with Tukey’s multiple comparisons test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38690566), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for MED24 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MED24
Alternate Names
cofactor required for Sp1 transcriptional activation, subunit 4, 100kDa, CRSP100, DRIP100CRSP complex subunit 4, hTRAP100, KIAA0130CRSP4, mediator complex subunit 24ARC100, MGC8748, subunit 24 homolog, thyroid hormone receptor associated protein 4, Thyroid hormone receptor-associated protein 4, Thyroid hormone receptor-associated protein complex 100 kDa component, Trap100, TRAP100THRAP4, Vitamin D3 receptor-interacting protein complex 100 kDa component, vitamin D3 receptor-interacting protein complex component DRIP100
Gene Symbol
MED24
UniProt
Additional MED24 Products
Product Documents for MED24 Antibody - BSA Free
Product Specific Notices for MED24 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...