MDR1/ABCB1 Antibody - BSA Free

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody - BSA Free [NB100-80870]

Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NB100-80870] - Staining of human kidney tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western Blot: MDR1/ABCB1 Antibody - BSA Free [NB100-80870] -

Acetylated androstano-arylpyrimidines do not suppress the efflux activity of multidrug-resistant cells by attenuating the expression of ABC transporters. (A) Relative mRNA levels of selected ABC transporters in Colo 320 cells treated with acetylated androstano-arylpyrimidines 10a, 10d, 10e, 10f, and 10g (in 20 μM concentration for 24 h) determined by RT-qPCR measurements and subsequent analyses by the delta deltaCt method using GAPDH as the reference gene. For primer sequences and concentrations, please refer to Supplementary Material Table S3. (B) Representative Western blot images and densitometric analysis of ABCB1 protein levels in Colo 320 cells following exposure to androstano-arylpyrimidine acetates (treatment with steroid compounds at 20 μM for 24 h) or Tunicamycin (600 nM, 24 h). The equal loading of protein samples was verified by probing the membrane with an anti-actin antibody. C = Control, Tun = Tunicamycin. In both panels, bar graphs represent mean +/- SD values. Fisher’s LSD test, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001 ns: non-significant. Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1999-4923/15/2/584), licensed under a CC-BY license. Not internally tested by Novus Biologicals.