Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

PerCP (Excitation = 490 nm, Emission = 675 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 750835

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Lgr5/GPR49 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line, CHO derived recombinant human Lgr5/GPR-49
Met1-Ile560
Accession # O75473

Specificity

Detects human Lgr5/GPR-49 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Lgr5/GPR49 Antibody (750835) [PerCP]

Lgr5/GPR49 Antibody (750835) [PerCP]

Detection of human Lgr5/GPR49by Flow Cytometry

Signal from unstained and stained samples. HCT116 were stained with 5 uL of LGR5-PerCP antibody for 30 minutes at 4 C. Image from a verified customer review.
Lgr5/GPR49 Antibody (750835) [PerCP]

Flow Cytometry: Mouse Lgr5/GPR49 mAb (750835) [PerCP] [FAB80781C]

Flow Cytometry: Mouse Lgr5/GPR49 mAb (750835) [PerCP] [FAB80781C] - Treated and untreated SVF cells were stained with 5 uL for 30 minutes at 4C. Image from a verified customer review.

Applications for Lgr5/GPR49 Antibody (750835) [PerCP]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product MAB80781. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Reviewed Applications

Read 2 reviews rated 5 using FAB80781C in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Lgr5/GPR49

Leucine-rich repeat-containing LGR5, also known as GPR49, is an Orphan-A G-protein coupled receptor with an unknown ligand. GPR49 is a stem cell marker of the intestinal epithelium and the hair follicule, and is often expressed in the brain, skeletal muscle, placenta, and spinal cord. Because LGR5 is typically found in mouse hair follicles it may potentially be used to regrow hair in mice. GPR49/LGR5 antibodies are useful tools for research on hair loss and hair follicule development.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Additional Lgr5/GPR49 Products

Product Documents for Lgr5/GPR49 Antibody (750835) [PerCP]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Lgr5/GPR49 Antibody (750835) [PerCP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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