Importin alpha 2/KPNA2 Antibody (24GB5955)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-50975

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NBP3-50975-100ul
NBP3-50975-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB5955

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Importin alpha 2/KPNA2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Importin alpha 2/KPNA2 (Uniprot: P52292)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Importin alpha 2/KPNA2 Antibody (24GB5955)

Importin alpha 2/KPNA2 Antibody (24GB5955)

Immunocytochemistry/ Immunofluorescence: Importin alpha 2/KPNA2 Antibody (24GB5955)[NBP3-50975] -

Immunocytochemical staining of HepG2 cells with Importin alpha 2/KPNA2 antibody (1:1,000). Nuclei were stained blue with DAPI; Karyopherin subunit alpha 2 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Importin alpha 2/KPNA2 Antibody (24GB5955)

Flow Cytometry: Importin alpha 2/KPNA2 Antibody (24GB5955)[NBP3-50975] -

Flow cytometric analysis of Importin alpha 2/KPNA2expression in HepG2 cells using Importin alpha 2/KPNA2 antibody (1:2,000). Green, isotype control; red, karyopherin subunit alpha 2.
Importin alpha 2/KPNA2 Antibody (24GB5955)

Western Blot: Importin alpha 2/KPNA2 Antibody (24GB5955)[NBP3-50975] -

Western blotting analysis using Importin alpha 2/KPNA2 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
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Applications for Importin alpha 2/KPNA2 Antibody (24GB5955)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Importin alpha 2/KPNA2

The import of proteins into the nucleus is a process that involves at least 2 steps. The first is an energy-independent docking of the protein to the nuclear envelope and the second is an energy-dependent translocation through the nuclear pore complex. Imported proteins require a nuclear localization sequence (NLS) which generally consists of a short region of basic amino acids or 2 such regions spaced about 10 amino acids apart. Proteins involved in the first step of nuclear import have been identified in different systems. These include the Xenopus protein importin and its yeast homolog, SRP1 (a suppressor of certain temperature-sensitive mutations of RNA polymerase I in Saccharomyces cerevisiae), which bind to the NLS. KPNA2 protein interacts with the NLSs of DNA helicase Q1 and SV40 T antigen and may be involved in the nuclear transport of proteins. KPNA2 also may play a role in V(D)J recombination

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Gene Symbol

KPNA2

Additional Importin alpha 2/KPNA2 Products

Product Documents for Importin alpha 2/KPNA2 Antibody (24GB5955)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Importin alpha 2/KPNA2 Antibody (24GB5955)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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