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Key Product Details

Species Reactivity

Human, Mouse, Rhesus Macaque

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Summary for IL-17/IL-17A Antibody - BSA Free

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the center of mature human IL-17. The immunogen is located within amino acids 50-100 of IL-17.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (84%), Rat: (81%). Use in Rhesus Macaque reported in scientific literature (PMID:32277724).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IL-17/IL-17A Antibody - BSA Free

Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Analysis of 1 ng of recombinant human IL-17 with IL-17 antibody at 1 ug/ml.
Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP1-76337] - IL-17 in mouse colon tissue with IL-17 antibody at 2 ug/ml.
Western Blot: IL-17/IL-17A Antibody [NBP1-76337] - Loading: 10 ug of lysates per lane. Antibodies: IL-17 4887, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Exposure: 1 min Lane 1: 1 ug/mL Lane 2: 2 ug/mL

Applications for IL-17/IL-17A Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Immunohistochemistry

1-5 ug/ml

Immunohistochemistry-Paraffin

1-5 ug/ml

Western Blot

0.125-2 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 7 publications using NBP1-76337 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IL-17/IL-17A

Interleukin 17 (IL-17) is a family of pro-inflammatory cytokines produced by activated T cells and is thought to have a major role in the initiation and perpetuation of rheumatoid arthritis. IL-17 regulates the activities of NF-kappaB and mitogen-activated protein kinases such as ERK and JNK. In addition, IL-17 stimulates the expression of IL-6 and cyclooxygenase-2 and enhances the production of nitric oxide. IL-17-producing T helper cells (TH-17 cells) have been the subject of much attention due to the importance of IL-17 in the pathogenesis of autoimmune inflammation. Because of its role in autoimmune diseases, it is thought that targeting the production and action of IL-17 would be beneficial therapeutically in these diseases.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Product Documents for IL-17/IL-17A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for IL-17/IL-17A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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