Key Product Details

Species Reactivity

Mouse

Applications

ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 50101

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all IL-17/IL-17A Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-17
Thr22-Ala158
Accession # Q62386

Specificity

Detects mouse IL-17 in ELISAs and Western blots. In direct ELISAs, 100% reactivity with recombinant mouse (rm) IL-17A/IL-17F heterodimer, 50% cross-reactivity with recombinant human IL-17, and 25% cross-reactivity with recombinant canine IL-17 is observed. No cross-reactivity with rmIL-17B, rmIL-17C, rmIL-17D, rmIL-17E, or rmIL-17F is observed. In Western blots, cross-reactivity with recombinant rat IL-17A is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for IL-17/IL-17A Antibody (50101) [DyLight 550]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-17/IL-17A

Interleukin 17 (IL-17) is a pro-inflammatory cytokine produced by TCRalpha/beta+ CD4 CD8 thymocytes, as well as by activated CD4+ and CD4+ CD45RO+ memory T cells (1). IL-17 stimulates the expression of TNFalpha, IL-1 beta, IL-6, and IL-8 (2). In addition, IL-17 is involved in the induction of inducible nitric oxide synthase and cyclooxygenase 2 in chondrocytes (3). High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis (4).

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Gene Symbol

IL17A

Additional IL-17/IL-17A Products

Product Documents for IL-17/IL-17A Antibody (50101) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for IL-17/IL-17A Antibody (50101) [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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