ICAM-3/CD50 Antibody (Cal 3.34) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # BBA28CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, CyTOF-ready
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # Cal 3.34
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant human ICAM‑3/CD50 Fc Chimera
Specificity
Detects human ICAM‑3/CD50 in Western blots. ICAM‑3/CD50 was found not to recognize CHO cells expressing the human ICAM-1 protein. ICAM‑3/CD50 was found to recognize CHO cells expressing the human ICAM-3 protein. The epitope for ICAM‑3/CD50 is located on domain 4 of the human ICAM-3 molecule.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for ICAM-3/CD50 Antibody (Cal 3.34) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for ICAM-3/CD50 Antibody (Cal 3.34) [CoraFluor™ 1]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: ICAM-3/CD50
Long Name
Intercellular Adhesion Molecule 3
Alternate Names
CD50, ICAM3
Gene Symbol
ICAM3
Additional ICAM-3/CD50 Products
Product Documents for ICAM-3/CD50 Antibody (Cal 3.34) [CoraFluor™ 1]
Product Specific Notices for ICAM-3/CD50 Antibody (Cal 3.34) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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