Huntingtin Antibody (23GB6285)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51390

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NBP3-51390-100ul
NBP3-51390-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB6285

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Huntingtin Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Huntingtin (Uniprot: P42858)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Huntingtin Antibody (23GB6285)

Huntingtin Antibody (23GB6285)

Immunocytochemistry/ Immunofluorescence: Huntingtin Antibody (23GB6285)[NBP3-51390] -

Immunocytochemical staining of HepG2 cells with Huntingtin antibody (1:1,000). Nuclei were stained blue with DAPI;Huntingtin was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Huntingtin Antibody (23GB6285)

Flow Cytometry: Huntingtin Antibody (23GB6285)[NBP3-51390] -

Validation of Huntingtin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Huntingtin antibody (1:2,000) and analyzed using BD flow cytometer.
Huntingtin Antibody (23GB6285)

Flow Cytometry: Huntingtin Antibody (23GB6285)[NBP3-51390] -

Flow cytometric analysis of Huntingtinexpression in HepG2 cells using Huntingtin antibody (1:2,000). Green, isotype control; red, Huntingtin.
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Applications for Huntingtin Antibody (23GB6285)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Huntingtin

Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15) gene. Huntington's disease is a neurodegenerative disorder caused by a mutation on the HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. The mutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulated by proteolytic cleavage with caspases and calpains, producing N-terminal polyQ fragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity of mutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophic factor (BDNF), a pro-survival factor for striatal neurons. This suggests restoring wild-type Htt level and increasing BDNF expression level can be therapeutic treatment for Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine protease product of death-gene for human, suggesting HD might be a disorder of inappropriate apoptosis (4).

Long Name

Huntingtin

Alternate Names

HD, HTT, IT15

Gene Symbol

HTT

Additional Huntingtin Products

Product Documents for Huntingtin Antibody (23GB6285)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Huntingtin Antibody (23GB6285)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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