Detects human ZNF206 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ZNF24 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human ZNF206 Antibody

ZNF206 in BG01V Human Embryonic Stem Cells.

ZNF206 was detected in immersion fixed BG01V human embryonic stem cells using Goat Anti-Human ZNF206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4020) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with tubulin (green). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human ZNF206 Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed undifferentiated BG01V human embryonic stem cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human ZNF206

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNF206

ZNF206 (zinc finger protein 206; also ZFP206) is an 80 kDa SCAN-zinc finger transcription factor that is active in embryonic stem cells and appears to positively regulate transcriptional regulators that maintain stem cell pluripotency. Human ZNF206 is 725 amino acids in length. It contains one SCAN box (aa 1‑71) that mediates oligomerization, followed by a proline-rich region (aa 104‑175) and 14 consecutive C2H2-type zinc fingers (aa 292‑719). Mouse ZNF206 apparently has multiple isoforms, none of which are well characterized. The human gene may also show alternate splicing. Human ZNF206 is 72% aa identical to mouse ZNF206.

Long Name

Zinc Finger Protein 206/Zinc Finger and SCAN Domain Containing 10

Alternate Names

Zfp206, ZSCAN10

Entrez Gene IDs

84891 (Human); 332221 (Mouse); 302962 (Rat)

Gene Symbol

ZSCAN10

UniProt

Q96SZ4

Additional ZNF206 Products

Product Documents for Human ZNF206 Antibody

Product Datasheets

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COA

SDS

Download SDS

Product Specific Notices for Human ZNF206 Antibody

For research use only

Citations
Reviews

Human ZNF206 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF4020

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity