Human ZBP1/DLM-1/DAI Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6309T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human ZBP1/DLM-1/DAI
Ala2-Ile429
Accession # Q9H171
Ala2-Ile429
Accession # Q9H171
Specificity
Detects human ZBP1/DLM-1/DAI in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human ZBP1/DLM-1/DAI Alexa Fluor® 594-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: ZBP1/DLM-1/DAI
ZBP1 (Z-DNA binding protein 1; also DAI and DLM-1) is a 15-60 kDa cytosolic detector of intracellular DNA. In a TOLL-independent manner, ZBP1 detects cytoplasmic dsDNA and initiates INF-beta production. It does so by binding DNA, followed by multimerization and recruitment of TBK1 and IRF3. This is accompanied by a parallel interaction with RIP-1 and -3 that stimulates NF kappaB activity and cytokine production. Human ZBP1 is 429 amino acids (aa) in length. It contains two DNA-binding Z-regions (aa 6-72 and 103-168) plus a third DNA-binding region that likely lies in the region between aa 170-250. Multiple splice forms exist. One shows a deletion of aa 12-86, a second shows a 63 aa substitution for aa 87-429, a third contains a 24 aa substitution for aa 225-429, a fourth reveals a deletion of aa 87-109, while a fifth and sixth show 82 and 16 aa substitutions for aa 365-429, respectively. Full length human ZBP1 shares only 47% aa identity with mouse ZBP1.
Long Name
Zipcode-binding Protein 1
Alternate Names
DAI, DLM-1
Gene Symbol
ZBP1
UniProt
Additional ZBP1/DLM-1/DAI Products
Product Specific Notices for Human ZBP1/DLM-1/DAI Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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