Western blot shows human plasma and lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human ZAG by Simple Western^TM.

Simple Western lane view shows lysates of human prostate tissue, loaded at 0.2 mg/mL. A specific band was detected for ZAG at approximately 59 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human ZAG Monoclonal Antibody (Catalog # MAB4764). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human ZAG Antibody

Application

Recommended Usage

Simple Western

10 µg/mL
Sample: Human prostate tissue

Western Blot

1 µg/mL
Sample: Human plasma and human prostate tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZAG

ZAG (zinc-alpha ₂-glycoprotein; also ZA2G) is a 40 kDa, secreted member of the MHC class I family of proteins. It is produced by adipocytes and various epithelial cells that generate exocrine-type secretions. ZAG is reported to stimulate lipid breakdown and thus may play a role in lipid homeostasis. Mature human ZAG is 278 amino acids (aa) in length. It contains one MHC class I antigen region (aa 26‑201) and a C2‑type Ig‑like domain (aa 207‑292). Two alternate splice forms exist; one shows a 66 aa substitution for the C‑terminal 30 aa, and a second shows a nine Lys substitution for aa 151‑298. Mature human ZAG is 60% aa identical to mouse ZAG.

Long Name

Zinc alpha-2-Glycoprotein

Alternate Names

AZGP1, ZA2G, ZNGP1

Entrez Gene IDs

563 (Human); 12007 (Mouse); 25294 (Rat)

Gene Symbol

AZGP1

UniProt

P25311

Additional ZAG Products

Product Documents for Human ZAG Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ZAG Antibody

For research use only

Citations
Reviews

Human ZAG Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4764

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ZAG Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ZAG Antibody

Detection of Human ZAG by Western Blot.

Detection of Human ZAG by Simple WesternTM.