Human WIZ Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11743

R&D Systems, part of Bio-Techne
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MAB11743-100
MAB11743-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1110627

View all WIZ Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # O95785

Specificity

Detects a synthetic peptide specific for human WIZ around amino acid 1100 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for Human WIZ Antibody

Detection of WIZ in MG-63 Human Cell Line.

WIZ was detected in immersion fixed MG-63 human osteosarcoma cell line using Mouse Anti-Human WIZ Monoclonal Antibody (Catalog # MAB11743) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human WIZ Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed MG-63 human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIZ

Widely interspaced zinc finger motifs (WIZ) is an epigenetic regulatory protein with a molecular weight of approximately 95 kDa. This protein plays a critical role in chromatin remodeling and transcriptional regulation by interacting with key epigenetic modifiers such as G9a/GLP and other chromatin-associated complexes. WIZ is ubiquitously expressed across various tissues and is essential for maintaining proper gene expression patterns during development and differentiation. It has been implicated in processes such as neuronal development, where it facilitates neuron-specific gene activation and repression, and in safeguarding chromatin architecture. Dysregulation of WIZ expression or function contributes to pathological conditions, including various neuropsychiatric disorders and cancer, where its role in epigenetic silencing can influence tumorigenesis and disease progression. Mutations in the WIZ gene are associated with cognitive impairments and developmental delay syndromes, suggesting its importance in neuroplasticity and brain development. As a key mediator of chromatin dynamics, WIZ serves as a promising target for research into epigenetic therapies and biomarkers of disease.

References

  1. Mozzetta C, Pontis J, Ait-Si-Ali S. Functional Crosstalk Between Lysine Methyltransferases on Histone Substrates: The Case of G9A/GLP and Polycomb Repressive Complex 2. Antioxid Redox Signal. 2015 Jun 1;22(16):1365-81. doi: 10.1089/ars.2014.6116. Epub 2014 Dec 19. PMID: 25365549; PMCID: PMC4432786.
  2. Justice M, Carico ZM, Stefan HC, Dowen JM. A WIZ/Cohesin/CTCF Complex Anchors DNA Loops to Define Gene Expression and Cell Identity. Cell Rep. 2020 Apr 14;31(2):107503. doi: 10.1016/j.celrep.2020.03.067. PMID: 32294452; PMCID: PMC7212317.
  3. Simon JM, Parker JS, Liu F, Rothbart SB, Ait-Si-Ali S, Strahl BD, Jin J, Davis IJ, Mosley AL, Pattenden SG. A Role for Widely Interspaced Zinc Finger (WIZ) in Retention of the G9a Methyltransferase on Chromatin. J Biol Chem. 2015 Oct 23;290(43):26088-102. doi: 10.1074/jbc.M115.654459. Epub 2015 Sep 3. PMID: 26338712; PMCID: PMC4646261.

Long Name

WIZ zinc finger

Alternate Names

ZNF803

Entrez Gene IDs

58525 (Human); 22404 (Mouse); 314598 (Rat)

Gene Symbol

WIZ

UniProt

O95785 (Mouse)

Additional WIZ Products

Product Documents for Human WIZ Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human WIZ Antibody

For research use only

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