HUVECS cells treated with 5 ug/mL Brefeldin A for 5 hours were stained with Human vWF-A1 (RUO grade Caplacizumab Biosimilar) Antibody (Catalog # MAB10591, Filled histogram) or isotype control antibody Llama IgG (open histogram), followed by Goat anti Alpaca Rhodamine Red. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: HUVEC Human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: vWF-A1

Caplacizumab is a humanized VHH immunoglobulin that binds to the A1 domain of the large von Willdebrand factor resulting in the inhibition of the interaction of glycoprotein GPIb-IX-V receptor on platelet surface. The result of neutralizing of the von Willedebrand factor is reduced platelet aggregation. Caplacizumab has been approved for the treatment of acute thrombotic thrombocytopenic purpura (aTTP), and has been shown to reduce the occurrence of thromboembolic events.

Long Name

von Willebrand Factor A1 Domain

Alternate Names

vWFA1

Entrez Gene IDs

7450 (Human)

Gene Symbol

VWF

Additional vWF-A1 Products

Product Documents for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10591

Key Product Details

Validated by

Species Reactivity

Applications

Label

Antibody Source

View all vWF-A1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human vWF-A1 (Research grade Caplacizumab Biosimilar) Antibody

Detection of vWF-A1 in HUVECS cells by Flow Cytometry.