Human VISTA/B7-H5/PD-1H Antibody

Name: Human VISTA/B7-H5/PD-1H Antibody
Brand: R&D Systems
SKU: MAB11704

R&D Systems, part of Bio-Techne | Catalog # MAB11704

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Product Datasheet / COA / SDS

Recombinant Monoclonal Antibody.

Key Product Details

Species Reactivity:	Human
Immunogen:	Synthetic Peptide Accession # Q9H7M9
Applications:	Simple Western, Western Blot
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 3180B
Specificity:	Detects a synthetic peptide specific for human VISTA around amino acid 100 in Direct ELISA.

View Full Technical Details for Human VISTA/B7-H5/PD-1H Antibody

Technical Data
Citations / Reviews

Applications for Human VISTA/B7-H5/PD-1H Antibody

	Recommended Concentration	Sample
Western Blot	2 µg/mL	Human placenta
Simple Western	2-10 µg/mL	Human placenta

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human VISTA/B7-H5/PD-1H Antibody

	Western Blot Detection of Human VISTA/B7-H5/PD-1H by Western Blot. Western Blot shows lysates of human placenta. PVDF membrane was probed with 2 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for VISTA/B7-H5/PD-1H at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Simple Western Detection of Human VISTA/B7-H5/PD-1H by Simple Western^TM. Left: Simple Western lane view shows lysates of human placenta, loaded at 0.1 mg/ml. A specific band was detected for VISTA/B7-H5/PD-1H at approximately 59 kDa (as indicated) using both 2 µg/ml and 10 µg/ml of Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) followed by HRP-conjugated Goat Anti-Rabbit Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. Right: Simple Western electropherogram showing the same Rabbit Anti-Human VISTA/B7-H5/PD-1H Monoclonal Antibody (Catalog # MAB11704) tested at 2 µg/ml (blue line) and 10 µg/ml (green line) in human placenta.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VISTA/B7-H5/PD-1H

Platelet Receptor Gi24, also known as VISTA (V-domain Ig suppressor of T cell activation), B7-H5, B7H5, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162 aa extracellular region with one V-type Ig-like domain and a 96 aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment plus a 25-30 kDa membrane-bound fragment. Over aa 33-194, human Gi24 shares 70% and 67% aa identity with mouse and rat Gi24, respectively.

Item	Value
Entrez Gene IDs	64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names	4632428N05Rik, B7-H5, B7H5, C10orf54, Dies1, Gi24, PD-1H, PD1H, PP2135, SISP1, VISTA, VSIR, chromosome 10 open reading frame 54, platelet receptor Gi24, stress induced secreted protein 1

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Human VISTA/B7-H5/PD-1H Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11704

Key Product Details

Applications for Human VISTA/B7-H5/PD-1H Antibody

Scientific Data Examples for Human VISTA/B7-H5/PD-1H Antibody

Western Blot

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: VISTA/B7-H5/PD-1H

Customer Reviews for Human VISTA/B7-H5/PD-1H Antibody

Product Documents for Human VISTA/B7-H5/PD-1H Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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