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Human VE‑Cadherin PE‑conjugated Antibody
Catalog # FAB9381P | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 123413
Product Summary for Human VE‑Cadherin PE‑conjugated Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151
Asp48-Gln593
Accession # P33151
Specificity
Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin and no cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human VE‑Cadherin PE‑conjugated Antibody
Detection of VE‑Cadherin in HUVEC Human Cells by Flow Cytometry.
HUVEC human umbilical vein endothelial cells were stained with Mouse Anti-Human VE-Cadherin PE-conjugated Monoclonal Antibody (Catalog # FAB9381P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). Cells were stained in a buffer containing Ca2+and Mg2+. View our protocol for Staining Membrane-associated Proteins.Applications for Human VE‑Cadherin PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells stained in a buffer containing Ca2+ and Mg2+
Sample: HUVEC human umbilical vein endothelial cells stained in a buffer containing Ca2+ and Mg2+
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Published Applications
Read 12 publications using FAB9381P in the following applications:
Formulation, Preparation and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: VE-Cadherin
Vascular endothelial (VE)-Cadherin (VE-CAD), also called 7B4 and Cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-Cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-Cadherin proteins share approximately 74% homology.
References
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
- Carmeliet, P. et al. (1999) Cell 98:147.
- Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Vascular Endothelium Cadherin
Alternate Names
Cadherin-5, CD144, CDH5, VECadherin
Gene Symbol
CDH5
UniProt
Product Documents for Human VE‑Cadherin PE‑conjugated Antibody
Product Specific Notices for Human VE‑Cadherin PE‑conjugated Antibody
For research use only
Citations for Human VE‑Cadherin PE‑conjugated Antibody (12)
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Citations are publications that use Bio-Techne products. Selected citations for Human VE‑Cadherin PE‑conjugated Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryJ Kelleher et al. (2019-10-31), Patient-Specific iPSC Model of a Genetic Vascular Dementia Syndrome Reveals Failure of Mural Cells to Stabilize Capillary Structures Stem Cell Reports, 2019-113(5):817-831.
PMID: 31680059 -
Species: Human
Sample Types: Whole Cells
Applications: ICCS Masuda et al. (2018-07-07), Preparation of iPS cell-derived CD31+ endothelial cells using three-dimensional suspension culture Regen Ther, 2018-09(0):1-9.
PMID: 30525069 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryC Maroun-Eid et al. (2018-01-05), Effect of intensive multifactorial treatment on vascular progenitor cells in hypertensive patients PLoS ONE, 2018-013(1):e0190494.
PMID: 29304136 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryLan Huang (2016-10-21), Somatic GNAQ Mutation is Enriched in Brain Endothelial Cells in�Sturge-Weber Syndrome Pediatr. Neurol, 2016-10(0).
PMID: 27919468 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryHuang L et al. (2015-01-01), Glucose transporter 1-positive endothelial cells in infantile hemangioma exhibit features of facultative stem cells. Stem Cells, 2015-033(1):133-45.
PMID: 25187207 -
Species: Human
Sample Types: Whole Cells
Applications: ICC,Flow CytometryWhite M et al. (2013-01-01), Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells, 2013-031(1):92-103.
PMID: 23079999 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryPelosi E et al. (2012-12-04), Human haemato-endothelial precursors: cord blood CD34+ cells produce haemogenic endothelium. PLoS ONE, 2012-17(12):e51109.
PMID: 23226561 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryAhrens I et al. (2011-08-12), Successful In Vitro Expansion and Differentiation of Cord Blood Derived CD34+ Cells into Early Endothelial Progenitor Cells Reveals Highly Differential Gene Expression. PLoS ONE, 2011-06(8):e23210.
PMID: 21858032 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryRicci-Vitiani L et al. (2010-11-21), Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells. Nature, 2010-1468(7325):824-8.
PMID: 21102434 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryNarazaki M et al. (2008-02-13), Sulfated polysaccharides identified as inducers of neuropilin-1 internalization and functional inhibition of VEGF165 and semaphorin3A. Blood, 2008-0111(8):4126-36.
PMID: 18272814 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryPorat Y et al. (2006-12-01), Isolation of an adult blood-derived progenitor cell population capable of differentiation into angiogenic, myocardial and neural lineages. Br. J. Haematol., 2006-1135(5):703-14.
PMID: 17052254 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryPigott R et al. (1991-07-01), Structural and functional studies of the endothelial activation antigen endothelial leucocyte adhesion molecule-1 using a panel of monoclonal antibodies. J. Immunol., 1991-0147(1):130-5.
PMID: 1711068
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