Human VE‑Cadherin Antibody

Name: Human VE‑Cadherin Antibody
Brand: R&D Systems
SKU: MAB9381
Rating: 5 (2 reviews)

Catalog # MAB9381 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (12)

Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 123413

Product Summary for Human VE‑Cadherin Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151

Specificity

Detects human VE-Cadherin in Western blots. In Western blots, 25% cross-reactivity with recombinant mouse VE‑Cadherin andno cross‑reactivity with recombinant human (rh) Cadherin-17 or rhP-Cadherin is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human VE‑Cadherin Antibody

Detection of Human VE‑Cadherin by Western Blot.

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VE‑Cadherin in HUVEC Cells.

VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human VE‑Cadherin Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells stained in buffer containing Ca²⁺ and Mg²⁺

Immunocytochemistry

0.5-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using MAB9381 in the following applications:

Immunocytochemistry/Immunofluorescence (2 Reviews)

Published Applications

Read 12 publications using MAB9381 in the following applications:

Flow Cytometry (3 Publications)
ICC (10 Publications)

Formulation, Preparation and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.

References

Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
Overduin, M. et al. (1995) Science 267:386.
Takeichi, M. (1991) Science 251:1451.
Nose, A. et al. (1987) EMBO J. 6:3655.
Carmeliet, P. et al. (1999) Cell 98:147.
Gory-Faure, S. et al. (1999) Development 126:2093.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Gene Symbol

CDH5

UniProt

P33151

Product Documents for Human VE‑Cadherin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human VE‑Cadherin Antibody

For research use only

Citations
Reviews

Citations for Human VE‑Cadherin Antibody (12)

Showing 1 - 10 of 12 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human VE‑Cadherin Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

S Summer et al. (2022-12-15), Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices PLoS ONE, 2022-117(12):e0278895.
PMID: 36520838
Species: Human
Sample Types: Whole Cells
Applications: ICC

XY Cui et al. (2022-11-08), FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro International Journal of Molecular Sciences, 2022-123(22).
PMID: 36430180
Species: Human
Sample Types: Whole Cells
Applications: ICC

R Daum et al. (2020-03-23), Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Cells, 2020-09(3).
PMID: 32210018
Species: Human
Sample Types: Whole Cells
Applications: ICC

Y Kiyan et al. (2019-09-19), Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling Sci Rep, 2019-09(1):13591.
PMID: 31537875
Species: Human
Sample Types: Whole Cells
Applications: ICC

T Neri et al. (2019-04-26), Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis Nat Commun, 2019-010(1):1929.
PMID: 31028265
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: ICC

BL Ecker et al. (2018-10-02), Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis Cancer Discov, 2018-19(1):82-95.
PMID: 30279172
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Li et al. (2018-06-13), Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018-00(0):9636897187803.
PMID: 29895168
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICC

K Kulhankova et al. (2018-02-20), The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function Infect. Immun., 2018-00(0).
PMID: 29229737
Species: Human
Sample Types: Whole Cells
Applications: ICC

JC Bramley et al. (2017-06-21), A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier mSphere, 2017-02(3).
PMID: 28656176
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Gabriela Odent Grig (2016-12-12), Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016-10(0).
PMID: 27943613
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Lindsley RC et al. (2008-07-03), Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell, 2008-03(1):55-68.
PMID: 18593559
Species: Human
Sample Types: Whole Cells
Applications: ICC

Rajesh M et al. (2007-03-23), Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol., 2007-0293(1):H610-9.
PMID: 17384130

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Showing 1 - 10 of 12 publications Showing All

Customer Reviews for Human VE‑Cadherin Antibody (2)

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