Human VE‑Cadherin Antibody
Catalog # MAB9381 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human VE‑Cadherin Antibody
Immunogen
Asp48-Gln593
Accession # P33151
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human VE‑Cadherin Antibody
Detection of Human VE‑Cadherin by Western Blot.
Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.VE‑Cadherin in HUVEC Cells.
VE-Cadherin was detected in immersion fixed HUVEC cells using Mouse Anti-Human VE-Cadherin Monoclonal Antibody (Catalog # MAB9381) at 0.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human VE‑Cadherin Antibody
CyTOF-ready
Flow Cytometry
Sample: HUVEC human umbilical vein endothelial cells stained in buffer containing Ca2+ and Mg2+
Immunocytochemistry
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Western Blot
Sample: HUVEC human umbilical vein endothelial cells
Reviewed Applications
Read 2 reviews rated 4.5 using MAB9381 in the following applications:
Published Applications
Read 12 publications using MAB9381 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VE-Cadherin
Vascular endothelial (VE)-cadherin (VE-CAD), also called 7B4 and cadherin‑5 (CDH5), is a member of the cadherin family of cell adhesion molecules. Cadherins are calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium-dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-cadherin proteins share approximately 74% homology.
References
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
- Carmeliet, P. et al. (1999) Cell 98:147.
- Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Human VE‑Cadherin Antibody
Product Specific Notices for Human VE‑Cadherin Antibody
For research use only
Citations for Human VE‑Cadherin Antibody (12)
Citations are publications that use Bio-Techne products. Selected citations for Human VE‑Cadherin Antibody include:
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Species: Human
Sample Types: Whole Cells
Applications: ICCS Summer et al. (2022-12-15), Mesenchymal stem cells support human vascular endothelial cells to form vascular sprouts in human platelet lysate-based matrices PLoS ONE, 2022-117(12):e0278895.
PMID: 36520838 -
Species: Human
Sample Types: Whole Cells
Applications: ICCXY Cui et al. (2022-11-08), FSAP Protects against Histone-Mediated Increase in Endothelial Permeability In Vitro International Journal of Molecular Sciences, 2022-123(22).
PMID: 36430180 -
Species: Human
Sample Types: Whole Cells
Applications: ICCR Daum et al. (2020-03-23), Fibronectin Adsorption on Electrospun Synthetic Vascular Grafts Attracts Endothelial Progenitor Cells and Promotes Endothelialization in Dynamic In Vitro Culture Cells, 2020-09(3).
PMID: 32210018 -
Species: Human
Sample Types: Whole Cells
Applications: ICCY Kiyan et al. (2019-09-19), Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling Sci Rep, 2019-09(1):13591.
PMID: 31537875 -
Species: Human
Sample Types: Whole Cells
Applications: ICCT Neri et al. (2019-04-26), Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis Nat Commun, 2019-010(1):1929.
PMID: 31028265 -
Species: Human
Sample Types: Whole Tissue,Whole Cells
Applications: ICCBL Ecker et al. (2018-10-02), Age-Related Changes in HAPLN1 Increase Lymphatic Permeability and Affect Routes of Melanoma Metastasis Cancer Discov, 2018-19(1):82-95.
PMID: 30279172 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryJ Li et al. (2018-06-13), Functional 3D Human Liver Bud Assembled from MSC-Derived Multiple Liver Cell Lineages Cell Transplant, 2018-00(0):9636897187803.
PMID: 29895168 -
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry,ICCK Kulhankova et al. (2018-02-20), The Superantigen Toxic Shock Syndrome Toxin-1 Alters Human Aortic Endothelial Cell Function Infect. Immun., 2018-00(0).
PMID: 29229737 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJC Bramley et al. (2017-06-21), A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier mSphere, 2017-02(3).
PMID: 28656176 -
Species: Human
Sample Types: Whole Cells
Applications: Flow CytometryGabriela Odent Grig (2016-12-12), Synergic effects of VEGF-A and SDF-1 on the angiogenic properties of endothelial progenitor cells J Tissue Eng Regen Med, 2016-10(0).
PMID: 27943613 -
Species: Mouse
Sample Types: Whole Cells
Applications: ICCLindsley RC et al. (2008-07-03), Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs. Cell Stem Cell, 2008-03(1):55-68.
PMID: 18593559 -
Species: Human
Sample Types: Whole Cells
Applications: ICCRajesh M et al. (2007-03-23), Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption. Am. J. Physiol. Heart Circ. Physiol., 2007-0293(1):H610-9.
PMID: 17384130
There are no citations that match your criteria.
Customer Reviews for Human VE‑Cadherin Antibody (2)
Customer Images
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: HUVEC human umbilical vein endothelial cellsSpecies: HumanVerified Customer | Posted 04/05/2022
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Human Coronary Artery Endothelial CellsSpecies: HumanVerified Customer | Posted 09/10/2021
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