Human VE‑Cadherin Antibody Best Seller

Catalog # AF938 | R&D Systems, Inc. a Bio-Techne Brand
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AF938
AF938-SP
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Summary for Human VE‑Cadherin Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human VE-Cadherin
Asp48-Gln593
Accession # P33151

Specificity

Detects human VE-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse VE‑Cadherin is observed and less than 5% cross-reactivity with recombinant human E-Cadherin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human VE‑Cadherin Antibody

Detection of Human VE-Cadherin antibody by Western Blot.

Detection of Human VE‑Cadherin by Western Blot.

Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VE-Cadherin antibody in HUVEC Cells by Immunocytochemistry (ICC).

VE‑Cadherin in HUVEC Cells.

VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human VE‑Cadherin by Simple WesternTM.

Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for VE‑Cadherin at approximately 162 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human VE‑Cadherin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Simple Western

12.5 µg/mL
Sample: HUVEC human umbilical vein endothelial cells

Western Blot

0.25 µg/mL
Sample: HUVEC human umbilical vein endothelial cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using AF938 in the following applications:

Published Applications

Read 22 publications using AF938 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VE-Cadherin

Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.

References

  1. Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
  2. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  3. Overduin, M. et al. (1995) Science 267:386.
  4. Takeichi, M. (1991) Science 251:1451.
  5. Nose, A. et al. (1987) EMBO J. 6:3655.
  6. Carmeliet, P. et al. (1999) Cell 98:147.
  7. Gory-Faure, S. et al. (1999) Development 126:2093.

Long Name

Vascular Endothelium Cadherin

Alternate Names

Cadherin-5, CD144, CDH5, VECadherin

Entrez Gene IDs

1003 (Human); 12562 (Mouse)

Gene Symbol

CDH5

UniProt

P33151

Product Documents for Human VE‑Cadherin Antibody

Certificate of Analysis

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Product Specific Notices for Human VE‑Cadherin Antibody

For research use only

Citations for Human VE‑Cadherin Antibody (22)

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Customer Reviews for Human VE‑Cadherin Antibody (1)

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  • Human VE-Cadherin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: HMVEC human microvascular endothelial cells
    Species: Human
    Verified Customer | Posted 06/09/2021
    Human VE‑Cadherin Antibody AF938

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