Human VE‑Cadherin Antibody Best Seller
Catalog # AF938 | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Summary for Human VE‑Cadherin Antibody
Immunogen
Asp48-Gln593
Accession # P33151
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human VE‑Cadherin Antibody
Detection of Human VE‑Cadherin by Western Blot.
Western blot shows lysate of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VE-Cadherin at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.VE‑Cadherin in HUVEC Cells.
VE-Cadherin was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human VE-Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Detection of Human VE‑Cadherin by Simple WesternTM.
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for VE‑Cadherin at approximately 162 kDa (as indicated) using 12.5 µg/mL of Goat Anti-Human VE‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human VE‑Cadherin Antibody
CyTOF-ready
Flow Cytometry
Sample: HUVEC human umbilical vein endothelial cells
Immunocytochemistry
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Simple Western
Sample: HUVEC human umbilical vein endothelial cells
Western Blot
Sample: HUVEC human umbilical vein endothelial cells
Reviewed Applications
Read 1 review rated 5 using AF938 in the following applications:
Published Applications
Read 22 publications using AF938 in the following applications:
Formulation, Preparation and Storage
Purification
Reconstitution
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: VE-Cadherin
Vascular endothelial (VE) - cadherin (VE-CAD), also called 7B4 and cadherin-5, is a member of the cadherin family of cell adhesion molecules. Cadherins are
calcium‑dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. VE-Cadherin has been shown to play important roles in vasculogenesis and angiogenesis. VE-cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. Human VE-cadherin is a 784 amino acid (aa) residue protein with a 25 aa signal sequence and a 759 aa propeptide. The mature protein begins at amino acid 48 and has a 546 aa extracellular domain, a 27 aa transmembrane domain, and a 164 aa cytoplasmic domain. The human and mouse mature VE-CAD proteins share approximately 74% homology.
References
- Shimoyama, Y. et al. (1989) J. Cell Biol. 109:1787.
- Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
- Overduin, M. et al. (1995) Science 267:386.
- Takeichi, M. (1991) Science 251:1451.
- Nose, A. et al. (1987) EMBO J. 6:3655.
- Carmeliet, P. et al. (1999) Cell 98:147.
- Gory-Faure, S. et al. (1999) Development 126:2093.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Human VE‑Cadherin Antibody
Product Specific Notices for Human VE‑Cadherin Antibody
For research use only
Citations for Human VE‑Cadherin Antibody (22)
Citations are publications that use Bio-Techne products. Selected citations for Human VE‑Cadherin Antibody include:
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Species: Human
Sample Types: Whole Tissue
Applications: IHCStanton et al. (2023-08-17), Engineered 3D Immuno-Glial-Neurovascular Human Brain Model bioRxiv : the preprint server for biology
PMID: 37645757 -
Species: Human
Sample Types: Whole Cells
Applications: ICCRichards et al. (2023-08-07), SARS-CoV-2 infection of human pluripotent stem cell-derived vascular cells reveals smooth muscle cells as key mediators of vascular pathology during infection bioRxiv : the preprint server for biology
PMID: 37609322 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCKho et al. (2023-07-25), Argininosuccinate lyase deficiency causes blood-brain barrier disruption via nitric oxide-mediated dysregulation of claudin expression JCI insight
PMID: 37490345 -
Species: Human
Sample Types: Whole Cells
Applications: ICCHong et al. (2023-05-26), Polarized Mechanosensitive Signaling Domains Protect Arterial Endothelial Cells Against Inflammation bioRxiv : the preprint server for biology
PMID: 37292837 -
Species: Human
Sample Types: Whole Cells,Whole
Applications: ICCM Liu et al. (2023-01-20), Co-culture models of endothelial cells, macrophages, and vascular smooth muscle cells for the study of the natural history of atherosclerosis PLoS ONE, 2023-018(1):e0280385.
PMID: 36662769 -
Species: Human
Sample Types: Whole Tissue
Applications: RNAScopeChoudhury et al. (2023-01-01), NOTCH3 drives meningioma tumorigenesis and resistance to radiotherapy bioRxiv
PMID: 37503127 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCH Kim et al. (2022-11-02), Reactive astrocytes transduce inflammation in a blood-brain barrier model through a TNF-STAT3 signaling axis and secretion of alpha 1-antichymotrypsin Nature Communications, 2022-113(1):6581.
PMID: 36323693 -
Species: Human
Sample Types: Whole Cells
Applications: ICCAM Schroeder et al. (2022-10-14), Nascent polypeptide-Associated Complex and Signal Recognition Particle have cardiac-specific roles in heart development and remodeling PloS Genetics, 2022-118(10):e1010448.
PMID: 36240221 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Abello et al. (2022-08-19), Peristaltic pumps adapted for laminar flow experiments enhance in vitro modeling of vascular cell behavior The Journal of Biological Chemistry, 2022-00(0):102404.
PMID: 35988646 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Saume et al. (2021-07-15), LDLR expression in the cochlea suggests a role in endolymph homeostasis and cochlear amplification Hearing research, 2021-0409(0):108311.
PMID: 34311268 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,IHCYG Gete et al. (2021-06-04), Mechanisms of angiogenic incompetence in Hutchinson-Gilford progeria via downregulation of endothelial NOS Aging Cell, 2021-00(0):e13388.
PMID: 34086398 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCP Mora et al. (2020-11-30), Genetic disruption of the Blood Brain Barrier leads to protective barrier formation at the Glia Limitans PLoS Biol, 2020-118(11):e3000946.
PMID: 33253145 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Ohmes et al. (2020-09-21), Effect of Enzymatically Extracted Fucoidans on Angiogenesis and Osteogenesis in Primary Cell Culture Systems Mimicking Bone Tissue Environment Mar Drugs, 2020-018(9).
PMID: 32967359 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJW Blanchard et al. (2020-06-08), Reconstruction of the human blood-brain barrier in vitro reveals a pathogenic mechanism of APOE4 in pericytes Nat. Med., 2020-026(6):952-963.
PMID: 32514169 -
Species: Human
Sample Types: Whole Cells
Applications: ICCF Roudnicky et al. (2020-03-03), Identification of a combination of transcription factors that synergistically increases endothelial cell barrier resistance Sci Rep, 2020-010(1):3886.
PMID: 32127614 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCM Riddell et al. (2018-12-17), aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability Nat Commun, 2018-19(1):5357.
PMID: 30559384 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-FrK Nonomura et al. (2018-11-27), Mechanically activated ion channel PIEZO1 is required for lymphatic valve formation Proc. Natl. Acad. Sci. U.S.A., 2018-10(0).
PMID: 30482854 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Xu et al. (2017-02-21), Vascular CXCR4 expression promotes vessel sprouting and sensitivity to sorafenib treatment in hepatocellular carcinoma Clin. Cancer Res, 2017-00(0).
PMID: 28223275 -
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot(2016-05-19), DeepCAGE transcriptomics identify HOXD10 as transcription factor regulating lymphatic endothelial responses to VEGF-C J Cell Sci, 2016-00(0).
PMID: 27199372 -
Species: Mouse
Sample Types: Whole Cells
Applications: Flow CytometryWang J et al. (2013-10-31), MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus. Arterioscler Thromb Vasc Biol, 2013-134(0):99.
PMID: 24177325 -
Species: Human
Sample Types: Whole Cells
Applications: ICCNiwa A et al. (2011-07-27), A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors. PLoS ONE, 2011-06(7):e22261.
PMID: 21818303 -
Species: Human
Sample Types: Whole Cells
Applications: ICCKelly MA et al. (2009-02-12), Signaling hierarchy regulating human endothelial cell development. Arterioscler. Thromb. Vasc. Biol., 2009-029(5):718-24.
PMID: 19213939
There are no citations that match your criteria.
Customer Reviews for Human VE‑Cadherin Antibody (1)
Customer Images
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: HMVEC human microvascular endothelial cellsSpecies: HumanVerified Customer | Posted 06/09/2021
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