Human Use1/UBE2Z Alexa Fluor™ Plus 680-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8154AFP680
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Specificity
Detects human USE1/UBE2Z in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: Use1/UBE2Z
Uba6-specific E2 Conjugating Enzyme 1 (Use1), also known as Ubiquitin-conjugating Enzyme E2Z (UBE2Z), is a 38 to 43 kDa member of the Ubiquitin-conjugating (E2) enzyme family that is highly expressed in human placenta, pancreas, spleen, and testis. Use1 has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin. It functions specifically with the UBE1L2/UBA6 Ubiquitin-activating (E1) enzyme, rather than the UBE1 E1 enzyme, for Ubiquitin conjugation. Expression is enriched in the cytoplasm, where it mediates the ubiquitination of RGS4 and RGS5 via the N-end rule proteolytic pathway. Use1 also functions with UBE1L2/UBA6 to mediate the conjugation of HLA F-associated Transcript 10 (FAT10), a Ubiquitin-like protein, and is auto-FAT10ylated in response to TNF-alpha and IFN-gamma. Full-length human Use1 is 354 amino acids (aa) and shares 100% sequence identity with mouse and rat Use1. A potential smaller isoform lacks aa 1-108.
Additional Use1/UBE2Z Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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