Detects human ULBP‑4/RAET1E in direct ELISAs and Western blots. In Western blots, approximately 20% cross‑reactivity with recombinant human (rh) ULBP-2 is observed and no cross-reactivity with rhULBP‑1 or rhULBP-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Applications

Application

Recommended Usage

CyTOF-ready

Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Background: ULBP-4/RAET1E

ULBP-4 (cytomegalovirus glycoprotein UL16 binding protein 4), also called RAET1E (retinoic acid early transcript 1E), Letal (lymphocyte effector cell toxicity activating ligand) and NKG2DL4 (NKG2D ligand 4), is a 40-50 kDa member of the ULBP/RAET1 family of cell surface proteins that function as ligands for NKG2D (1‑6). While most family members are GPI-anchored, only ULBP-4/RAET1E and ULBP-5/RAET1G express a transmembrane form (1, 4, 6, 7). Human ULBP-4 mRNA encodes 263 amino acids (aa) including a 30 aa signal sequence, a 195 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 15 aa cytoplasmic sequence. A soluble 35 kDa form diverges at aa 208 and is thought to antagonize the transmembrane form (5). Other potential splice variants of 220, 227 and 280 aa are transmembrane proteins (8). Within the ECD, ULBP-4 shares 34-41% aa sequence identity with family members (1, 7). Rodent NKG2D ligands Rae-1 alpha-epsilon are, like human ULBP and MIB proteins, distantly related to MHC class I proteins, but none of the families share significant sequence identity (2, 4). Low expression of ULBP‑4 mRNA is found in normal tissues, with high expression variably reported in the small intestine (3) and skin (4). Expression is stimulated by TNF-alpha and down‑regulated by retinoic acid (3). ULBP-4 is abnormally expressed on most colon cancer and some other tumor cell lines and virus-infected peripheral blood cells (3, 6). ULBP-4 binds and costimulates NKG2D-expressing effector cells including NK cells, NKT cells, gamma delta T cells, and CD8⁺ alpha beta T cells, activating cytolytic activity and/or cytokine production (3, 4, 7). In some gamma delta T cells, direct ULBP-4 binding to both TCR gamma delta and NKG2D has been demonstrated (6). ULBP-4 is also thought to function as a minor histocompatibility antigen in humans (1).

References

Radosavljevic, M. et al. (2002) Genomics 79:114.
Kondo, M. et al. (2010) Immunogenetics 62:441.
Conejo-Garcia, J.R. et al. (2003) Cancer Biol. Ther. 2:446.
Chalupny, N.J. et al. (2003) Biochem. Biophys. Res. Commun. 305:129.
Cao, W. et al. (2007) J. Biol. Chem. 282:18922.
Kong, Y. et al. (2009) Blood 114:310.
Bacon, L. et al. (2004) J. Immunol. 173:1078.
Cao, W. et al. (2008) Int. Immunol. 20:981.

Long Name

UL16 Binding Protein-4

Alternate Names

LETAL, N2DL-4, N2DL4, NKG2DL4, RAET1E, RL-4, ULBP4

Entrez Gene IDs

135250 (Human); 379043 (Mouse); 292461 (Rat)

Gene Symbol

RAET1E

UniProt

Q8TD07 (Human)

Additional ULBP-4/RAET1E Products

Product Documents

Product Datasheets

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COA

SDS

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Product Specific Notices

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Human ULBP-4/RAET1E Alexa Fluor™ Plus 680-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6285AFP680

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Specificity

Clonality

Host

Isotype

Applications

CyTOF-ready

Flow Cytometry

Immunohistochemistry

Western Blot

Background: ULBP-4/RAET1E

References

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional ULBP-4/RAET1E Products

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices

CONTACT INFORMATION

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